渤海湾海水养殖杜氏盐藻培养条件优化  

Culture Condition Optimization of Dunaliella Salina Utilizing Seawater from Bohai Bay

作　　者：才金玲[1] 刘洁[1] 王雨 张鑫志 邱琦[2] 关月 CAI Jin-ling;Liu Jie;WANG Yu;ZHANG Xin-zhi;QIU Qi;GUAN Yue(College of Chemical Engineering and Materials Science,Tianjin University of Science and Technology,Tianjin 300457,China;Tianjin Changlu Hanggu Saltworks Co.Ltd,Tianjin 300480,China)

机构地区：[1]天津科技大学化学工程与材料科学学院,天津市卤水化学工程与资源生态利用重点实验室,天津300457 [2]天津长芦汉沽盐场有限责任公司,天津300480

出　　处：《盐科学与化工》2022年第12期28-37,共10页Journal of Salt Science and Chemical Industry

基　　金：天津市高等学校大学生创新创业训练项目(202110057169,202210057068)。

摘　　要：文章以杜氏盐藻为实验材料,对杜氏盐藻的培养进行单因素实验,针对碳源、氮源、磷源、盐度、钙离子和镁离子的种类及含量对该藻种生长及β-胡萝卜素、蛋白质和总糖等营养物质积累的影响。确定了各因素影响之下的最佳培养条件,根据各因素的最佳浓度对培养基进行优化,并针对优化后的培养基进行盐藻的培养和营养成分测定。结果表明:优化后的培养基对盐藻生长有显著的促进作用,盐藻的细胞密度最高可达(4.00±0.17)×10^(6) cells/mL,色素含量(叶绿素a、叶绿素b和β-胡萝卜素)分别达到了(18.36±0.66)μg/mL、(6.08±0.27)μg/mL和(7.80±0.43)μg/mL;蛋白质积累达到(2.74±0.16)mg/mL;总糖积累量为(2.27%±0.08%),均显著高于原培养基的细胞密度及营养物质积累量。Dunaliella salina is a large and versatile organism with high yield of glycerin andβ-carotene,and has great potential as an excellent feedstuff and raw materials for chemical and pharmaceutical products.In this article,the single factor experiment was carried out on the culture of Dunaliella salina.The effects of the types and contents of carbon source,nitrogen source,phosphorus source,salinity,calcium ion and magnesium ion on the growth and accumulation of nutrients such asβ-carotene,protein and total sugar of algae species were studied.The optimal culture conditions under the influence of various factors were determined,and the medium was optimized according to the optimal concentration of each factor,and the culture and nutrient composition of Dunaliella salina were determined for the optimized medium.The results showed that:The optimized medium significantly promoted the growth of Dunaliella salina,and the cell density of salina reached(4.00±0.17)×10^(6) cells/mL.The pigment contents(chlorophyll A,chlorophyll B andβ-carotene)were(18.36±0.66)μg/mL,(6.08±0.27)μg/mL and(7.80±0.43)μg/mL,respectively.Protein accumulation reached(2.74±0.16)mg/mL.The total sugar accumulation was 2.27%±0.08%,which were significantly higher than the cell density and nutrient accumulation of the original medium.

关 键 词：杜氏盐藻 培养条件优化 Β-胡萝卜素 

分 类 号：S937.3[农业科学—渔业资源]