复合菌固体发酵蛋壳粉制备乳酸钙  

Preparation of calcium lactate from eggshell powder by solid fermentation with compound bacteria

作　　者：赵奉龙 莫庭鸣 文璨 颜鸿宾 ZHAO Fenglong;MO Tingming;WEN Can;YAN Hongbin(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《发酵科技通讯》2022年第4期222-225,共4页Bulletin of Fermentation Science and Technology

摘　　要：为降低废弃鸡蛋壳对环境的污染以及避免废弃鸡蛋壳中大量的钙元素被浪费,提出了一种将鸡蛋壳中的碳酸钙转化为乳酸钙的方法。该方法以蛋壳粉为实验原料,使用嗜酸小球菌和枯草芽孢杆菌以固体发酵的方式生产乳酸钙;实验中以发酵产物中乳酸钙的质量分数为指标,通过单因素实验对蛋壳粉添加量、葡萄糖添加量、发酵培养基含水量、发酵时间和接种量等发酵条件进行优化。结果表明:在蛋壳粉添加量为8%、葡萄糖添加量为5%、含水量为50%、接种量为18%和发酵时间为96 h的条件下,发酵产物中乳酸钙的质量分数可以达到6.09%。In order to reduce the environmental pollution caused by waste egg shells and avoid the waste of a large amount of calcium in waste egg shells,we proposed a method to convert calcium carbonate in egg shells into calcium lactate.In our method,eggshell powder was used as experimental raw material,and Micrococcus acidophilus and Bacillus subtilis were used to produce calcium lactate by solid fermentation.During the experiment,the mass fraction of calcium lactate was taken in the fermentation product as the index.The fermentation conditions such as the addition of eggshell powder,the addition of glucose,the water content of fermentation medium,fermentation time and inoculation amount were optimized through single factor experiment.The results showed that under the conditions of 8% eggshell powder,5% glucose,50% water content,18% inoculum and 96 h fermentation time,the mass fraction of calcium lactate in the fermentation product reached 6.09%.

关 键 词：复合菌 固体发酵 乳酸钙 

分 类 号：S816.71[农业科学—饲料科学]