高效液相色谱-荧光检测法同时测定人参归脾丸中4种黄曲霉毒素  

Simultaneous determination of content of four Aflatoxins Ginseng spleen- invigorating bolus by HPLC-FLD

作　　者：李正刚 谢秋红[2] 邵大志[1] 王丹彧 赵磊[1,3] 马彧 LI Zheng-gang;XIE Qiu-hong;SHAO Da-zhi;WANG Dan-yu;ZHAO Lei;MA Yu(Department of Traditional Chinese Medicine,Siping Institute for Food and Drug Control,Siping,Jilin 136000,China;不详)

机构地区：[1]四平市食品药品检验所中药室,吉林四平136000 [2]四平市中心医院 [3]长春中医药大学药学院

出　　处：《中国卫生工程学》2022年第5期714-716,720,共4页Chinese Journal of Public Health Engineering

基　　金：吉林省科技发展重点研发医药健康领域(NO.20210204151YY)。

摘　　要：目的 建立免疫亲和柱净化柱后光化学衍生高效液相色谱-荧光检测法同时测定人参归脾丸中黄曲霉毒素(aflatoxi, AF)B、B、G、G的含量。方法 样品采用70%甲醇作为提取溶剂,经免疫亲和柱净化、高效液相色谱分离、光化学柱后衍生后,通过荧光检测器测定其中AF的含量。结果 AFB的线性范围为0.010 4~0.052 0 ng(r=0.999 9)、AFB的线性范围为0.003 8~0.019 0 ng(r=0.999 8)、AFG的线性范围为0.010 8~0.054 0 ng(r=0.999 8)、AFG的线性范围为0.003 8~0.019 0 ng(r=0.999 8),均线性关系良好,回收率在89.68~101.44%之间,RSD≤3.5%。结论 该方法操作简便,灵敏度高、重复性好、结果准确,可用于人参归脾丸中AF的测定。Objective This paper aims to establish the contamination method of AFB,B,Gand Gin Ginseng spleen-invigorating bolus by immunoaffinity column clean-up and HPLC-FLD with post-column photochemical derivatization.Methods After extraction with 70% Methanol and purification by immunoaffinity columns, AFB,B,Gand Gin samples were analyzed by HPLC-FLD with post-column photochemical derivatization.Results The linearity of AFBwas at 0.010 4-0.052 0 ng(r=0.999 9), AFBwas at 0.003 8-0.019 0 ng(r=0.999 8), AFGwas at 0.010 8-0.054 0 ng(r=0.999 8), AFGwas at 0.003 8-0.019 0 ng(r=0.999 8). The average recoveries were within 89.68-101.44%, with RSD≤3.5%.Conclusion The above method has demonstrated convenient operation, good repeatability, highsensitity and accuracy.This method is suitable for the determination of AF in Ginseng spleen-invigorating bolus.

关 键 词：免疫亲和柱 光化学衍生 高效液相色谱-荧光检测器 人参归脾丸 黄曲霉毒素 

分 类 号：R065[医药卫生]