低温暂养对珍珠龙胆石斑鱼存活、非特异性免疫及抗氧化指标的影响  

Effect of low-temperature acclimation on survival,non-specific immune and antioxidant indexes of Epinephelus fuscoguttatus♀×E.lanceolatus♂

作　　者：袁仲瑾 岑剑伟[2,3] 李来好 杨贤庆[2] 黄卉[2] 魏涯[2] 郝淑贤[2] 赵永强[2] 王悦齐[2] 林织[4] YUAN Zhongjin;CEN Jianwei;LI Laihao;YANG Xianqing;HUANG Hui;WEI Ya;HAO Shuxian;ZHAO Yongqiang;WANG Yueqi;LIN Zhi(College of Food Sciences&Technology,Shanghai Ocean University,Shanghai 201306,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Aquatic Processing,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;Dalian Polytechnic University/Collaborative Innovation Center of Seafood Deep Processing,Dalian 116034,China;Guangdong Shunxin Ocean Fishery Group Co.Ltd.,Yangjiang 529800,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]中国水产科学研究院南海水产研究所/农业农村部水产品加工重点实验室,广东广州510300 [3]大连工业大学/海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034 [4]广东顺欣海洋渔业集团有限公司,广东阳江529800

出　　处：《南方水产科学》2022年第6期118-126,共9页South China Fisheries Science

基　　金：广州市科技计划项目(202002030199);国家现代农业产业技术体系资助(CARS-46);海南省自然科学基金面上项目(321MS099);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2020CY03);广东省现代农业产业技术体系海水鱼产业创新项目(2019KJ143);中国水产科学研究院基本科研业务费专项资金(2020TD73);国家重点研发计划重点专项(2019YFD0901601);广东省企业科技特派员专项资助(GDKTP202135800)。

摘　　要：探究珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×E.lanceolatus♂)最佳暂养温度,可为该鱼的保活流通提供理论依据。以0.5~1.0℃·h^(−1)的速率将石斑鱼的暂养水温降至24、20、18和16℃,观察降温过程中石斑鱼的生理反应,测定暂养过程中的水质变化及鱼体保活时间、非特异性免疫和抗氧化等指标。结果显示,在24~16℃时石斑鱼的存活时间随暂养温度的降低呈上升趋势,水温维持在16℃时,水体中总氨氮(TAN)质量浓度增速较缓,鱼体存活时间最长(109 h)。不同水温下暂养48 h后,鱼体非特异性免疫和抗氧化能力均受到不同程度的影响。与暂养前相比,16℃组石斑鱼血清非特异性免疫指标中补体蛋白3(C3)、球蛋白(GLB)和总蛋白(TP)质量浓度显著上升,其余指标均无显著变化;肝脏组织中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性显著升高,谷胱甘肽过氧化物酶(GPx)活性显著降低,但明显高于其他温度组,丙二醛(MDA)水平显著低于其他温度组。16℃暂养不仅能保持较好的水质,同时也能降低鱼体代谢水平,维持机体内环境稳态,从而降低氧化应激反应造成的损伤,适合作为珍珠龙胆石斑鱼的暂养和运输温度。Investigating the best acclimation temperature conditions for Epinephelus fuscoguttatus♀×E.lanceolatus♂can provide a theoretical basis for its survival and circulation.We decreased the water temperature to 24,20,18 and 16℃at the rate of 0.5^(−1).0℃·h^(−1),then recorded the physiological responses and survival time,as well as the changes of water quality,non-specific immune index and antioxidant indicator.The results show that the survival time of the grouper increased with the decrease of temperature within the range of 24^(−1) 6℃.When the water temperature was maintained at 16℃,the concentration of total ammonia nitrogen(TAN)increased slowly,and the survival time was the longest,up to 109 h.The non-specific immune and antioxidant parameters changed after the grouper being cultured at different temperatures for 48 h.Compared with the control group,the contents of complement protein 3(C3),globulin(GLB)and total protein(TP)increased in 16℃group significantly,while the other indexes had no significant changes.The activities of superoxide dismutase(SOD)and catalase(CAT)in the liver tissue in 16℃group increased significantly.However,the activity of glutathione peroxidase(GPx)decreased significantly,but higher than those of the other temperature groups.Compared with the other temperature treated groups,the content of malondialdehyde(MDA)decreased significantly.Thus,16℃temporary culture can not only maintain good water quality,but also reduce the metabolic level of fish and maintain the homeostasis of internal environment,so as to reduce the damage caused by oxidative stress reaction.It is suitable for temporary culture and transportation of Epinephelus fuscoguttatus♀×E.lanceolatus♂.

关 键 词：珍珠龙胆石斑鱼 低温暂养 非特异性免疫 抗氧化 

分 类 号：S983[农业科学—捕捞与储运]