生肌玉红膏对血栓性脉管炎大鼠血管平滑肌细胞凋亡、炎性因子表达的影响及机制  

Effects of Shengji Yuhong ointment on apoptosis of vascular smooth muscle cells and expression of inflammatory factors in rats with thromboangiitis

作　　者：王亚娜 宋晓聪[1] 吴静[1] 崔罗方 刘丽[1] 马文华[1] 赵秀雷 徐林丽 南桂英[1] 王慧玲[1] WANG Yana;SONG Xiaocong;WU Jing;CUI Luofang;LIU Li;MA Wenhua;ZHAO Xiulei;XU Linli;NAN Guiying;WANG Huiling(Teaching and Research Department,Cangzhou Medical College,Cangzhou 061001,China;不详)

机构地区：[1]沧州医学高等专科学校教研室,河北沧州061001 [2]沧州中心医院外科 [3]沧州医学高等专科学校内科护理教研室

出　　处：《山东医药》2022年第34期36-40,共5页Shandong Medical Journal

基　　金：沧州市科技计划项目(162302168)。

摘　　要：目的探究生肌玉红膏对血栓性脉管炎大鼠血管平滑肌细胞凋亡、炎性因子表达的影响及机制。方法取40只大鼠,随机分为正常组、模型组、血管内皮祖细胞(EPC)干预组、生肌玉红膏组,每组10只,除正常组外,均建立血栓性脉管炎模型,正常组与模型组尾静脉每天1次输注1 mL生理盐水,EPC干预组输注数量为5×105个EPC细胞后创面外敷生肌玉红膏,生肌玉红膏组创面外敷生肌玉红膏。将EPC细胞培养后观察细胞形态,检测IL-6、IL-8、TNF-α、TGF-β_(1)表达,采用免疫荧光法检测CD34、CD133,TUNEL法检测平滑肌细胞凋亡率,免疫印迹法检测内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)蛋白表达。结果模型组大鼠血清中IL-6、IL-8、TNF-α、TGF-β_(1)水平及平滑肌细胞数高于正常组(P均<0.05),EPC干预组以上指标均低于模型组(P均<0.05),生肌玉红膏组以上指标均高于EPC干预组(P均<0.05)。模型组大鼠血管组织中CD34、CD133及血管组织中VEGF、bFGF蛋白表达低于正常组(P均<0.05),EPC干预组以上指标高于模型组(P均<0.05),生肌玉红膏组以上指标低于EPC干预组(P均<0.05)。正常组大鼠生长良好;模型组大鼠血栓显著,可见大量炎性细胞浸润;EPC干预组血栓变化缓解,伴随少量炎性细胞,血管平滑肌细胞排列趋于整齐;生肌玉红膏组少量血栓,血管平滑肌细胞排列略显紊乱。模型组大鼠血管组织中CD34、CD133表达低于正常组(P均<0.05),EPC干预组以上指标高于模型组(P均<0.05),生肌玉红膏组CD34、CD133指标低于EPC干预组(P均<0.05)。与正常组比较,模型组血管组织中平滑肌细胞凋亡率高(P<0.05);与模型组比较,EPC干预组血管组织中平滑肌细胞凋亡率高(P均<0.05);与EPC干预组比较,生肌玉红膏组平滑肌细胞凋亡率较低(P均<0.05)。模型组大鼠血管组织中VEGF、bFGF蛋白表达低于正常组(P均<0.05),EPC干预组VEGF、bFGF蛋白表达高于模型组,生肌�Objective To explore the effects of Shengji Yuhong ointment on apoptosis of vascular smooth muscle cells and expression of inflammatory factors in rats with thromboangiitis and their mechanisms.Methods Forty rats were randomly divided into the normal group,model group,endothelial progenitor cells(EPCs)intervention group,and Shengji Yuhong ointment group,with 10 rats in each group.Except the normal group,thromboangiitis models were established.The tail vein normal saline(1 mL)was injected once a day in the normal group and model group;the rats were injected with 5×105 EPCs in the EPCs intervention group,and the wound surface was treated with Shengji Yuhong ointment;Shengji Yuhong ointment was applied on the wound surface in the Shengji Yuhong ointment group.We observed the morphology of EPCs after culture,and detected the interleukin-6(IL-6),IL-8,tumor necrosis factor-α(TNF-α),transforming growth factor-β1(TGF-β1).The expression of inflammatory factors,CD34 and CD133 were detected by immunofluorescence;the apoptosis rate of smooth muscle cells was detected by TUNEL;and the expression levels of vascular endothelial growth fac⁃tor(VEGF)and basic fibroblast growth factor(bFGF)protein were detected by immunoblotting.Results IL-6,IL-8,and TNF-αand the TGF-β1 in serum,and the number of smooth muscle cells was higher in the model group than in the nor⁃mal group(all P<0.05),the above indexes in the EPCs intervention group were lower than those in the model group(all P<0.05),and the above indexes in the Shengji Yuhong ointment group were higher than those in the EPCs intervention group(all P<0.05).The expression levels of CD34,CD133 and VEGF,bFGF protein in the vascular tissues of rats in the model group were lower than those in the normal group,the above indexes in the EPCs intervention group were higher than those in the model group(all P<0.05),and the above indexes in the Shengji Yuhong ointment group were lower than those in the EPCs intervention group(all P<0.05).The normal rats grew well;in the model group

关 键 词：血栓性脉管炎 生肌玉红膏 血管内皮细胞分化 血管平滑肌细胞 大鼠 

分 类 号：R543[医药卫生—心血管疾病]