产纤维素酶霉菌筛选及发酵条件优化  

Screening of Cellulase-Producing Fungi and Optimization of Fermentation Conditions

作　　者：干建松[1,2] GAN Jian-song(School of Grain and Food&Pharmacy,Jiangsu Vocational College of Finance&Economics,Huaian 223003,Jiangsu,China;School of Chemical Engineering&Technology,China University of Mining and Technology,Xuzhou 221116,Jiangsu,China)

机构地区：[1]江苏财经职业技术学院粮食与食品药品学院,江苏淮安223003 [2]中国矿业大学化工学院,江苏徐州221116

出　　处：《食品研究与开发》2022年第24期195-203,共9页Food Research and Development

基　　金：国家自然科学基金青年科学基金项目(21606097);淮安市重大成果转化项目(HAN201803);淮安市自然科学研究计划项目(HAB202068)。

摘　　要：从江苏淮安清江浦区柳树湾树林获取土样为菌种来源,以羧甲基纤维素钠(carboxymethyl cellulose sodium,CMC-Na)为唯一碳源,通过富集培养、初筛、复筛获得3株产纤维素酶能力较强的菌株(Strain-1、Strain-2、Strain-3,S-1、S-2、S-3),其中S-2产纤维素酶能力最强,其滤纸酶(filter paper enzyme,FPA)酶活力可达0.21 U/mL。对S-2菌株进行紫外(ultraviolet,UV)诱变,筛选获得菌株SUV2-1。通过单因素试验和响应面试验设计,对该菌株进行发酵培养基优化,得到该菌株产纤维素酶培养基配方:秸秆粉11.63 g/L,蛋白胨2.33 g/L、吐温-802.71 mL/L。利用上述培养基在摇瓶发酵条件下得到的FPA酶活力为0.45 U/mL,与优化前菌株的FPA酶活力相比,提高了51.15%;与诱变前的出发菌株相比,酶活力提高了113.00%。Three filamentous fungi strains[Strain-1(S-1),Strain-2(S-2),and Strain-3(S-3)]were obtained using enrichment culture,and two rounds of screening using soil samples obtained from Liushuwan forest(Qingjiangpu District,Jiangsu Province,China),with sodium carboxymethylcellulose(CMC-Na)as the only carbon source.Among the three strains,S-2 had the strongest cellulase production ability with an FPA activity of 0.21 U/mL.To improve the cellulase production ability of S-2,the strain was subjected to ultraviolet mutagenesis resulting in the strain SUV2-1.Through single-factor experimentation and response surface experimental design,the fermentation medium for the strain was optimized.The optimal medium for cellulase enzyme production from SUV2-1 contained straw powder(11.63 g/L),peptones(2.33 g/L),and Tween-80(2.71 mL/L).FPA activity obtained using this medium under shaking flask fermentation conditions was 0.45 U/mL.This represented an FPA activity increase of 51.15%compared with the strain prior to fermentation optimization and a 113.00%increase compared with the original strain prior to mutagenesis.

关 键 词：羧甲基纤维素钠 纤维素酶 紫外诱变 培养基配方 

分 类 号：TQ925[轻工技术与工程—发酵工程]