芸豆体外模拟消化液的抗氧化和α-淀粉酶抑制活性及其淀粉水解动力学  

Antioxidant andα-amylase inhibitory activities and starch hydrolysis kinetics of kidney bean by simulating digestion in vitro

作　　者：石娟 包鸿慧 张佩 张倜培 周睿 SHI Juan;BAO Hong-hui;ZHANG Pei;ZHANG Ti-pei;ZHOU Rui(College of Food Science and Technology,Hubei University of Arts and Science,Xiangyang,Hubei 441053,China)

机构地区：[1]湖北文理学院食品科技学院,湖北襄阳441053

出　　处：《食品与机械》2022年第11期20-26,共7页Food and Machinery

基　　金：国家自然科学基金项目(编号:31201333);湖北省教育厅科学研究项目(编号:B2020144);大学生创新创业训练计划项目(编号:X202210519157)。

摘　　要：目的:评价全芸豆食品的生理功效,开发不同花色芸豆功能性资源。方法:采用体外胃肠模拟消化模型,分析芸豆消化液总酚、总黄酮和酸性多糖含量、抗氧化活性与α-淀粉酶抑制活性,以及其淀粉水解动力学与葡萄糖扩散性。结果:总酚、总黄酮和酸性多糖含量、抗氧化能力及α-淀粉酶抑制能力为黑芸豆>红芸豆>白芸豆(P<0.05)。淀粉水解一级反应动力学速率、快速消化淀粉含量及消化液葡萄糖扩散速率为黑芸豆<红芸豆<白芸豆(P<0.05),且芸豆消化液活性组分含量与抗氧化活性呈显著正相关(P<0.05),与葡萄糖扩散速率呈显著负相关(P<0.05)。结论:芸豆消化液中富含酚类化合物与酸性多糖,具有良好的抗氧化活性与一定的α-淀粉酶抑制活性。Objective:To evaluate the physiological effects of whole kidney been food and develop functional resources of different color kidney beans.Methods:The contents of total polyphenols,flavonoids and acid polysaccharides,and antioxidant andα-amylase inhibitory activities of digestive solution and starch hydrolysis kinetics and glucose diffusion properties of three kidney bean were investigated through in vitro simulated gastrointestinal digestion.Results:The contents of total polyphenols,flavonoids and acid polysaccharides,and antioxidant andα-amylase inhibitory activities were black kidney bean>red kidney bean>white kidney bean(P<0.05).The kinetic rate of first order reaction of starch hydrolysis,rapidly digestible starch contents and glucose diffusion rate were black kidney bean<red kidney bean<white kidney bean(P<0.05).Additionally,the contents of bioactive components of kidney bean digestive juices were positively correlated with the antioxidant activities,but negatively correlated with the rates of glucose diffusion(P<0.05).Conclusion:The digestive juices of kidney bean were rich in phenolic compounds and exhibited excellent antioxidant activity and certainα-amylase inhibitory ability.

关 键 词：芸豆 体外模拟消化 抗氧化 α-淀粉酶 水解动力学 葡萄糖扩散 

分 类 号：TS201.2[轻工技术与工程—食品科学]