机构地区:[1]江苏省家禽遗传育种重点实验室,江苏省家禽科学研究所,江苏扬州225125 [2]佛山科技学院,广东佛山528225 [3]广西金陵农牧集团有限公司,南宁530000
出 处:《中国农业科学》2022年第23期4769-4780,共12页Scientia Agricultura Sinica
基 金:江苏省农业重大新品种创制项目(PZCZ201728);扬州市现代农业项目(YZ2021029);江苏现代农业(肉鸡)产业技术体系(JATS[2020]357);现代农业产业技术体系建设专项(CARS-41);江苏省农业自主创新基金(CX(21)2011-1);江苏省自然科学基金(BK20210955);江苏省种业振兴揭榜挂帅项目(JBGS(2021)107)。
摘 要:【目的】Wnt信号通路在动物皮肤毛囊的发生发育中具有重要作用,前期研究结果表明Wnt3a可能是影响鸡皮肤毛囊密度性状的重要候选基因。为进一步验证Wnt3a在皮肤毛囊生长发育中的作用,研究以金陵花鸡为研究对象,筛选Wnt3a SNPs,并分析其与毛囊密度性状的相关性,以期找到对皮肤毛囊密度有显著影响的分子标记,为培育屠体美观的屠宰型肉鸡的“分子编写育种”提供参考。【方法】采用PCR扩增直接测序法对Wnt3a的SNPs位点进行筛查,分析单个SNP标记与皮肤毛囊密度性状的相关性。采用Haploview软件分析这些SNP位点的连锁不平衡(LD)程度,并分析不同单倍型组合与毛囊密度性状的相关性。【结果】共发现14个SNP位点,在第2外显子发现1个SNP位点(SNP1),为同义突变;第2内含子发现4个突变位点(SNP2—SNP5),在第3内含子发现9个SNP位点(SNP6—SNP14)。卡方检验表明,第2外显子的1个突变位点(SNP1)和第2内含子的3个突变位点(SNP3—SNP5)的3个位点均处于哈代-温伯格平衡状态(P>0.05),第3内含子的9个SNPs(SNP6-SNP14)偏离哈代-温伯格平衡(P<0.05)。SNP1—SNP5的He均小于0.50,PIC均小于0.25,这5个SNP位点遗传多样性较低。第3内含子的9个突变位点,SNP6、SNP7、SNP9位点PIC<0.25,其他6个突变位点0.25<PIC<0.5,为中度多态。单标记关联分析表明,公、母鸡SNP2位点的AG基因型的毛囊密度显著高于GG基因型(P<0.05);母鸡SNP8位点的AA与GG基因型的毛囊密度显著高于AG基因型(P<0.05),在公鸡中3种基因型的毛囊密度差异不显著。14个SNPs连锁不平衡分析表明,SNP3、SNP4和SNP5的强连锁产生了2种单倍型,H1(GAT)频率为0.882和H2(TCC)频率为0.118;SNP6—SNP13之间的强连锁产生了5种单倍型,其中H1(ACATTATC)和H2(ACGTCCCA),H3(ACGTTCCA),H4(GTGCTATA),H5(ACGTCCCC),单倍型频率分别为0.469、0.275、0.123、0.113、0.014。SNP3、SNP4和SNP5连锁产生的2种单倍型组合后产生了3�【Objective】The Wnt signaling pathway plays an important role in the development of animal skin feather follicles.The results of previous studies indicated that the Wnt3a might be an important candidate gene that had effects on the chicken feather follicle density.In order to further verify the role of Wnt3a in the growth and development of skin feather follicle density,Wnt3a SNPs were screened and their association with feather follicle density would be analyzed in Jinling Hua chicken.The study could provide a reference for“breeding by molecular writing”of slaughter-type broilers with beautiful carcasses.【Method】The SNPs of Wnt3a gene were screened by PCR amplification and direct sequencing,and the correlation between a single SNP marker and skin feather follicle density traits was analyzed.Haploview software was used to analyze the degree of linkage disequilibrium(LD)of these SNP loci,and the correlation between different haplotype combinations and feather follicle density traits was also analyzed.【Result】A total of 14 SNP sites were found,and one SNP site(SNP1)was found in the second exon,which was a synonymous mutation.Four mutation sites(SNP2-SNP5)were found in the second intron,and 9 SNP sites(SNP6-SNP14)were found in the third intron.The chi-square test showed that one mutation site(SNP1)in the second exon and three mutation sites(SNP3-SNP5)in the second intron were all in the Hardy-Weinberg equilibrium(P>0.05),9 mutation sites(SNP6-SNP14)of the third intron deviated from Hardy-Weinberg equilibrium(P<0.05).The expected heterozygosity(He)of SNP1-SNP5 was less than 0.50,and the polymorphic information content(PIC)was less than 0.25.The genetic polymorphisms of these 5 SNP loci were low.The third intron had 9 mutation sites,PIC of SNP6,SNP7,SNP9 sites was less than 0.25,and the other 6 mutation sites were 0.25<PIC<0.5,which were moderately polymorphic.Single-marker association analysis showed that the number of skin feather follicle with SNP2 locus of AG genotype in males and females was signif
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...