海参蒸煮液对小鼠酒精性胃溃疡的预防作用  

Preventive Effect of Sea Cucumber Cooking Liquid on Alcoholic Gastric Ulcer in Mice

作　　者：蔡维震 张雷[1] 丁宁 王庆慧 胡炜 王静凤[1] Cai Weizhen;Zhang Lei;Ding Ning;Wang Qinghui;Hu Wei;Wang Jingfeng(Ocean University of China,Qingdao 266000,Shandong;Qingdao Malvern College,Qingdao 266000,Shandong;Shandong Haodangjia Ocean Development Co.Ltd.,Weihai 264200,Shandong)

机构地区：[1]中国海洋大学,山东青岛266000 [2]青岛墨尔文中学,山东青岛266000 [3]山东好当家海洋发展股份有限公司,山东威海264200

出　　处：《中国食品学报》2022年第11期173-180,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2018YFC0311203)。

摘　　要：目的:探究海参蒸煮液(SCCL)对急性酒精性胃溃疡小鼠的预防保护效果及作用机制。方法:采用85%乙醇灌胃诱导急性胃溃疡小鼠模型;评估小鼠胃组织形态、计算溃疡指数;H&E染色观察小鼠胃黏膜病理变化;测定氧化应激和炎症因子相关生化指标,评估SCCL对急性酒精性胃溃疡小鼠的抗氧化和抗炎能力;采用qRT-PCR法测定胃组织MAPK/NF-κB信号通路相关基因表达。结果:与模型组相比,SCCL组胃溃疡指数、胃黏膜细胞损伤程度显著降低,胃组织氧化应激因子、炎症因子趋于正常水平,并下调了MAPK/NF-κB相关基因的表达。结论:海参蒸煮液可通过保护小鼠胃黏膜,改善机体氧化应激、炎症水平等方面来预防保护酒精诱导的急性胃溃疡。Objective:To investigate the preventive and protective effect of SCCL on acute alcoholic gastric ulcer in mice and its mechanism.Methods:The mice model of acute gastric ulcer was established by intragastric administration of 85%ethanol after fasting.The macroscopic state of the gastric tissue was evaluated and the ulcer index was calculated.The pathological changes of gastric mucosa cells were observed by H&E staining.The biochemical indexes related to oxidative stress and inflammatory factors were measured to evaluate the anti-oxidation and anti-inflammatory ability of sea cucumber cooking liquid on mice with acute alcoholic gastric ulcer.The expression of MAPK/NF-κB signaling pathway related genes in gastric tissues was determined by qRT-PCR.Results:Compared with model group,gastric ulcer index and gastric mucosal cell damage degree were significantly improved in SCCL group,oxidative stress factor and inflammatory factor in gastric tissue tended to normal levels,and the expression of MAPK/NF-κB related genes were down-regulated.Conclusion:SCCL can prevent and protect alcohol-induced acute gastric ulcer by improving oxidative stress and inflammation levels in mice.

关 键 词：海参蒸煮液 酒精性胃溃疡 氧化应激 炎症因子 MAPK/NF-κB信号通路 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]