新型驱蚊产品中35种挥发性致敏芳香剂的筛查方法及挥发规律研究  

Study on Screening of 35 Volatile Al ergic Fragrances in New Mosquito Repel ent Products and Their Volatilization Patterns

作　　者：李红艳 胡玉霞 李长于 王瑾 LI Hong-yan;HU Yu-xia;LI Chang-yu;WANG Jin(Zhejiang Institute of Product Quality and Safety Science,Hangzhou 310018,China;Zhejiang Institute of Economics and Trade,Hangzhou 310018,China;Zhejiang Fangyuan Testing Group Co.,Ltd,Hangzhou 310018,China)

机构地区：[1]浙江省产品质量安全科学研究院,浙江杭州310018 [2]浙江经贸职业技术学院,浙江杭州310018 [3]浙江方圆检测集团股份有限公司,浙江杭州310018

出　　处：《分析测试学报》2022年第12期1758-1764,共7页Journal of Instrumental Analysis

基　　金：浙江省市场监督管理局科技计划项目(20210102);雏鹰计划重大培育项目(CY202109)。

摘　　要：基于顶空/气相色谱-串联质谱(HS/GC-MS/MS)建立了驱蚊贴、驱蚊扣、驱蚊手环等新型驱蚊产品中35种挥发性致敏芳香剂的分析方法。35种物质经中等极性色谱柱Agilent DB-17MS分离,采用电子轰击源(EI源)通过多反应监测(MRM)模式进行检测。考察了37个新型驱蚊样品在顶空温度分别为40℃和100℃时的筛查结果,40℃顶空条件下筛查出α-蒎烯、β-蒎烯和D-柠檬烯等14种致敏芳香剂,此外,100℃时还检出香叶醇、香芹酮等其它12种致敏芳香剂。以儿童用驱蚊贴和驱蚊扣为例,比较了不同顶空温度对各目标化合物响应强度的影响,结果表明,40℃时α-蒎烯、β-蒎烯和D-柠檬烯等8种致敏芳香剂的响应强度小于100℃时。对37个样品在40℃平衡温度下的半定量分析表明,α-蒎烯、β-蒎烯、D-柠檬烯等9种致敏芳香剂的检出率最高,其中α-蒎烯和D-柠檬烯的最高响应强度大于106。选择驱蚊贴和驱蚊扣进一步考察了α-蒎烯、β-蒎烯、D-柠檬烯、薄荷醇和樟脑5种致敏芳香剂在40℃下放置不同时间的挥发规律,发现α-蒎烯、β-蒎烯和D-柠檬烯放置12 h的响应强度为初始的20%~21%,薄荷醇和樟脑放置72 h后响应强度仍为初始的40%以上。该研究可为后续开展新型驱蚊产品中化学物质的含量测定和风险评估提供技术参考。A headspace/gas chromatography-tandem mass spectrometry(HS/GC-MS/MS)was established for the screening of 35 volatile allergic fragrances in different new mosquito repellent products.The new mosquito repellent products included mosquito repellent stickers,mosquito repellent buckles and mosquito repellent bracelets,etc.35 substances were separated by a medium polarity Agilent DB-17MS column and detected with electron impact ion source(EI)under multiple reactions monitoring(MRM)mode.Two different headspace equilibrium temperatures of 40 ℃ and 100 ℃ were selected.The screening results for 37 new mosquito repellent products showed significant differences at different temperatures.A total of 14 volatile allergic fragrances were detected at 40 ℃, including α-pinene, β-pinene, D-limonene, benzaldehyde, terpinolene,linalool,DL-menthol,D-camphor,linalyl acetate,citronellol,methyl salicylate,citral,β-caryophyllene and cinnamaldehyde.In addition,another 12 allergic fragrances at 100 ℃were found, including terpineol, geraniol, carvone, 4-methoxyphenol, 4-tert-butylphenol,trans-anethole,eugenol,benzalacetone,lily aldehyde,farnesol,α-amylcinnamyl alcohol and benzyl benzoate.Taking the mosquito repellent stickers and mosquito repellent buckles for children for examples,the response intensities of the target compounds at different headspace temperatures were semi-quantitatively determined.The results showed that the response intensities for the 8 volatile allergic fragrances at 40 ℃ were less than those at 100 ℃.37 samples were semi-quantitatively analyzed at the equilibrium temperature of 40 ℃.The results indicated that9 volatile allergic fragrances,i.e.α-pinene,β-pinene,D-limonene,benzaldehyde,terpinolene,linalool,DL-menthol,D-camphor and methyl salicylate were detected in most samples,in which the maximum response intensities for α-pinene and D-limonene were more than 106.The volatilization patterns for volatile allergic fragrances in new mosquito repellent products were also studied at 40 ℃ . The conten

关 键 词：顶空 气相色谱-串联质谱 驱蚊产品 致敏芳香剂 挥发规律 

分 类 号：O657.7[理学—分析化学] O657.63[理学—化学]