氧化胭脂虫红酸用于蘑菇中的铁离子检测  

Oxidized Carminic Acid for the Detection of Iron Ions in Mushroom

作　　者：刘兰香 李想 唐保山 谭瑞 徐涓 马金菊 张弘[1] LIU Lanxiang;LI Xiang;TANG Baoshan;TAN Rui;XU Juan;MA Jinju;ZHANG Hong(Key Laboratory of Breeding and Utilization of Resource Insects,Research Center of Engineering and Technology of Characteristic Forest Resources,National Forestry and Grassland Administration,Institute of Highland Forest Science,Chinese Academy of Forestry,Kunming 650233,China;College of Life Science,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]中国林业科学研究院高原林业研究所,国家林业和草原局特色森林资源工程技术研究中心,国家林业和草原局资源昆虫培育与利用重点实验室,云南昆明650233 [2]西南林业大学生命科学学院,云南昆明650224

出　　处：《食品科学》2022年第24期342-348,共7页Food Science

基　　金：中央级公益性科研院所基本科研业务费专项(CAFYBB2020SY029)。

摘　　要：为进一步开发胭脂虫红酸的荧光检测应用,以胭脂虫红酸为原料,高碘酸钾为氧化剂,通过乙醇/水热法一步合成水溶性可发射蓝色荧光的氧化胭脂虫红酸(oxidized carminic acid,OCA)。通过傅里叶变换红外光谱法和X射线光电子能谱分析表明OCA结构中含有共轭芳香环结构和丰富的含氧官能团,透射电子显微镜和动态散射光测试表明其平均粒径约为7.5 nm。光学测试表明,OCA的荧光发射位于380~620 nm区间,最大激发波长为336 nm,最大发射波长为445 nm;其荧光在pH 1~9的溶液和含有不同阴离子的盐溶液中稳定性良好;紫外-可见光测试显示其在波长225 nm处有最大吸收峰。OCA对铁离子(Fe^(3+))有良好的选择性,加入Fe^(3+)会导致其荧光强度明显降低,荧光发射峰积分面积减小率与Fe^(3+)质量浓度在11.2~56.0 mg/L范围内具有良好的线性关系,检出限为2.5 mg/L。应用结果表明,基于OCA作为荧光传感器的荧光检测方法用于实际样品中Fe^(3+)的检测,样品回收率在95.3%~110.4%之间,相对标准偏差在2.3%~4.6%之间,表明该方法可用于香菇、白菇和杏鲍菇等蘑菇中Fe^(3+)的含量检测,方法可靠。In order to further develop the application of carmininc acid(CA)for fluorescence detection,oxidized carminic acid(OCA),which is water soluble and emits blue fluorescence,was synthesized in one step by an ethanol-assisted hydrothermal method using carminic acid as the raw material and potassium periodate as the oxidant.Fourier transform infrared(FT-IR)spectroscopy and X-ray photoelectron spectroscopy(XPS)analysis showed that the structure of OCA contained conjugated aromatic rings and a large number of oxygen-containing functional groups,and transmission electron microscopy(TEM)and dynamic scattering light(DLS)indicated an average particle size of approximately 7.5 nm.Optical tests showed that the fluorescence emission of OCA appeared in the range of 380–620 nm,the maximum excitation wavelength was 336 nm,and the maximum emission wavelength was 445 nm.OCA showed excellent fluorescence stability in solutions at pH 1–9 and in different salt solutions containing anions.UV-visible absorption spectroscopy showed that its maximum absorption peak appeared at 225 nm.OCA had good selectivity for iron ions,which led to a significant decrease in its fluorescence intensity.The percentage of decrease in the fluorescence emission peak integrated area,(S_(0)–S)/S_(0),showed a good linear relationship with the mass concentration of iron ions in the range of 11.2–56.0 mg/L,and the limit of detection was 2.5 mg/L.Applying an OCA-based fluorescent sensor to detect the content of iron ions in mushrooms,the recoveries of this method was 95.3%–110.4%,with relative standard deviation(RSD)between 2.3%and 4.6%,indicating that the fluorescence detection method is reliable.

关 键 词：胭脂虫红酸 高碘酸钾 荧光检测 蘑菇 铁离子 

分 类 号：TS207.3[轻工技术与工程—食品科学] TQ617.1[轻工技术与工程—食品科学与工程]