Anti-lipid-oxidation effects and edible safety evaluation of the oil extracted by a supercritical CO_(2) process from coix seed fermented by Monascus purpureus  

作　　者：Haiying Zeng Anran Zhu Shengling He Mingjun Wu Muhammad Mazhar Anyan Wen Na Liu Likang Qin Song Miao 

机构地区：[1]Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China [2]School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China [3]Teagasc Food Research Centre,Moorepark,Fermoy,Co.Cork P61 C996,Ireland

出　　处：《Food Science and Human Wellness》2023年第4期1119-1127,共9页食品科学与人类健康（英文）

基　　金：supported by Natural Science Foundation of China(32260583);the Agriculture Committee of Guizhou Province[(2017)106&(2018)81];the Talent Introduction Program of Guizhou University[(2021)76].

摘　　要：The physicochemical properties and composition of coix seed oil produced by Monascus purpureus fermentation and supercritical CO_(2)extraction were determined.Anti-lipid-oxidation and edible safety were evaluated using a cholesterol-fish oil model,acute oral toxicity assay,and genetic toxicity assay in vitro and in vivo,respectively.The results show that the extraction oil from fermented coix seed(FCS-O)had good physicochemical quality and abundant active components with physiological function.In particular,γ-tocotrienol,γ-oryzanol,coixenolide and oleic acid concentrations reached 72.83μg/g,745.96μg/g,9.65 mg/g and 316.58 mg/100 g DW,respectively.The FCS-O exhibited higher antioxidant capability in inhibiting lipid oxidation and peroxidation.Compared to the blank control,the concentrations of 7-ketocholestreol and peroxide only were 8.42μg/mL and 16.16 mmol/kg at 168 h of oxidation(P<0.01).In addition,the FCS-O has been confirmed to be a very safe edible oil,with no acute toxicity(LD50>10 g/kg bw,considered actually non-toxic)and no induced mutagenicity,cytotoxicity or genotoxicity.These results serve as a good safety reference for future application of the oil from fermented coix seed.The development and utilization of this kind of oil will be beneficial as a food,food ingredient,nutritional supplement,or natural food antioxidant to promote good health function.

关 键 词：Coix seed oil Composition Edible safety MUTAGENICITY GENOTOXICITY 

分 类 号：TS224.4[轻工技术与工程—粮食、油脂及植物蛋白工程]