一株高产脂肪酶菌株WCO-9全基因组测序及比较基因组分析  

Whole Genome Sequencing and Comparative Genomic Analysis of a High-yield Lipase-producing Strain WCO-9

作　　者：张泽颖 范清锋 邓云峰 韦廷舟 周正富[2] 周建[1,3] 王劲[2] 江世杰[1,3] ZHANG Ze-ying;FAN Qing-feng;DENG Yun-feng;WEI Ting-zhou;ZHOU Zheng-fu;ZHOU Jian;WANG Jin;JIANG Shi-jie(College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081;Engineering Research Center of Biomass Materials,Ministry of Education,Southwest University of Science and Technology,Mianyang 621010)

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010 [2]中国农业科学院生物技术研究所,北京100081 [3]西南科技大学生物质材料教育部工程研究中心,绵阳621010

出　　处：《生物技术通报》2022年第10期216-225,共10页Biotechnology Bulletin

基　　金：四川省大学生创新创业训练计划项目(S202110619123);国家自然科学基金项目(31800061);四川省科技创新苗子工程(2021070)。

摘　　要：琼氏不动杆菌WCO-9是从油污污染的土壤中分离的一株高产脂肪酶菌株,所产脂肪酶具有显著优于对照同种菌株ATCC 17908的水解活性。为探究WCO-9菌株高效产酶机制,挖掘新型特异脂肪酶功能基因,采用Oxford Nanopore技术完成WCO-9菌株的全基因组测序,并对同属菌株进行比较基因组学分析。结果显示,脂肪酶产生菌WCO-9的基因组大小为3.19 Mb,GC含量38.62%,含有2929个编码基因,其中包含11个脂肪酶编码基因(1个特异基因)和3个脂肪酶分子伴侣基因;共线性分析也说明WCO-9菌株基因组与对照菌株ATCC 17908具有显著差异。WCO-9菌株全基因组序列分析,对该菌的高效产酶机制研究及新型脂肪酶特异基因挖掘具有重要意义,为后期高产脂肪酶工程菌的创制及工业化应用提供理论基础。Acinetobacter junii WCO-9 is a high-yield lipase-producing strain isolated from oil-contaminated soil.The lipase produced by this strain has significantly better hydrolysis activity than that of the control strain ATCC 17908.In order to explore the efficient enzyme production mechanism of WCO-9 strain and to discover specific lipase functional genes,ONT(Oxford Nanopore Technologies)was used to complete the whole genome sequencing of WCO-9 strain,and the comparative genomic analysis of the same strain was carried out.The results showed that the genome size of lipase-producing WCO-9 strain was 3.19 Mb,GC content was 38.62%,and there were 2929 coding genes,including 11 lipase-coding genes(1 specific gene)and 3 lipase molecular chaperones.The collinearity analysis also revealed that the WCO-9 strain was significantly different from the control strain ATCC 17908.The whole genome sequence analysis of WCO-9 strain will be of great significance for the study of high-efficiency enzyme production mechanism and the discovery of new lipase-specific genes,which provides a theoretical basis for the creation and industrial application of high-yield lipase engineering bacteria in the future.

关 键 词：脂肪酶 琼氏不动杆菌 全基因组测序 基因功能注释 比较基因组学 

分 类 号：Q936[生物学—微生物学]