紫色红曲霉色素与食药同源中药联合降脂作用  

Synergistic lipid-lowering of Monascus purpureus pigments and traditional Chinese medicine with food and drug homology

作　　者：吴勇 李文馨 杨华昊 邱俊辉 曹瑜 徐辉 乔代蓉 曹毅 WU Yong;LI Wenxin;YANG Huahao;QIU Junhui;CAO Yu;XU Hui;QIAO Dairong;CAO Yi(Microbiology and Metabolic Engineering of Key Laboratory of Sichuan Province,College of Life Sciences,Sichuan University,Chengdu 610065,China;Laboratory and Equipment Management Office,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学生命科学学院,微生物与代谢工程四川省重点实验室,成都610065 [2]四川大学实验室及设备管理处,成都610065

出　　处：《应用与环境生物学报》2022年第5期1297-1303,共7页Chinese Journal of Applied and Environmental Biology

基　　金：四川省科技厅项目(2021YJ0024);成都市科技局项目(2020-GH02-00037-HZ);国家自然科学基金项目(32071479)资助。

摘　　要：为探究紫色红曲霉色素与食药同源降脂中药(TCM)联用协同降血脂的可能性,进一步丰富红曲色素的降脂特性,为新型降血脂功能食品的制备奠定理论基础,从红曲中分离到一株不产Monacolin K的紫色红曲霉,经发酵条件优化后提取红曲色素,并测定红曲色素的体外抗氧化性;同时在传统降脂方中挑选合适的食药同源降脂中药进行新型降脂方复配,并通过HepG2细胞试验测定红曲色素与中药联用时对细胞总胆固醇(TC)和总甘油三酯(TG)含量及脂质代谢相关基因表达的影响.结果表明,红曲色素具有良好的体外抗氧化活性,对DPPH自由基、羟自由基、超氧阴离子自由基的清除率分别为88.1%、47.7%和37.5%.红曲色素与中药联用时对细胞内TC和TG含量的降低分别达到62%和66%,大于中药组(对TC和TG的降低分别为49%和57%)与色素组(对TC和TG的降低分别为7%和3%)两者降脂之和,说明红曲色素与中药联用在降血脂上具有协同作用.本研究表明红曲色素与中药联用制备新型降脂功能食品具有可行性,该结果可为高脂血症的治疗提供更多的选择.This study aimed to explore the possibility of the combined use of Monascus pigment and traditional Chinese medicine(TCM) with food and drug homology to synergistically lower blood lipids, further enrich the lipid-lowering properties of Monascus pigment and lay a theoretical foundation for the preparation of new lipid-lowering functional foods. In this study, a purple Monascus strain that did not produce monacolin K was isolated from red yeast rice. After the fermentation conditions were optimized, the pigment was extracted, and its in vitro antioxidant activity was determined. At the same time, suitable TCM with food and drug homology from traditional lipid-lowering prescriptions were selected to compound a new lipid-lowering prescription. HepG2cells were used to determine the effect of this prescription on total cholesterol, total triglyceride content, and the expression of lipid metabolism-related genes. The results of this study showed that Monascus pigment has good in vitro antioxidant activity, and the scavenging rates of DPPH free radicals, hydroxyl free radicals, and superoxide anion free radicals were 88.1%, 47.7%, and 37.5%, respectively. The combined use of Monascus pigment and TCM reduced the intracellular TC and TG content by 62% and 66%, respectively, which was greater than that of the sum of the TCM group(where reduction of TC and TG was 49 and 57%, respectively)and the pigment group(where reduction of TC and TG was 7 and 3%, respectively). This shows that the combination of Monascus pigment and TCM has a synergistic effect on lowering blood lipids. Therefore, the combination of Monascus pigment and TCM to prepare new lipid-lowering functional foods is feasible and can provide more options for the treatment of hyperlipidemia.

关 键 词：红曲色素 降脂中药 HepG2细胞模型 

分 类 号：TS201.4[轻工技术与工程—食品科学] R589.2[轻工技术与工程—食品科学与工程]