牛血清白蛋白与辛烯基琥珀酸淀粉酯糖基化产物改善谷维素的理化性质和生物可及度  

Improving the physicochemical property and bioavailability of γ-oryzanol by the glycosylation product of bovine serum albumin and octenyl succinate anhydride starch

作　　者：刘曼丽 钟金锋[1] 刘雄[1] 覃小丽[1] LIU Manli;ZHONG Jinfeng;LIU Xiong;QIN Xiaoli(College of Food Science,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学食品科学学院,重庆400715

出　　处：《食品与发酵工业》2022年第24期166-171,共6页Food and Fermentation Industries

基　　金：重庆市自然科学基金(cstc2019jcyj-msxmX0113)。

摘　　要：该研究利用干法美拉德反应制备牛血清白蛋白(bovine serum albumin,BSA)-辛烯基琥珀酸淀粉酯(octenyl succinate anhydride starch,OSAS)糖基化产物,构建负载谷维素(γ-oryzanol,GO)的糖基化产物的纳米粒子(BSA-OSAS-GO),探究其对GO性能及生物可及度的影响。结果表明,BSA-OSAS与GO的质量比为40∶1时,BSA-OSAS-GO的粒径为111.75 nm,其包埋率(84.46%)显著高于对照组[BSA-GO为62.78%,负载GO的BSA与OSAS物理共混(Mix-GO)为75.67%]。BSA-OSAS-GO的DPPH自由基清除率(55.44%)显著高于BSA-GO(48.32%)和Mix-GO(41.03%)。相比于对照组,BSA-OSAS-GO纳米粒子粒径仅增加了262.26 nm,有更好的抗消化效果,且能更有效地保护谷维素,经4℃贮藏15 d后GO残留率为84.45%。在体外消化180 min后,BSA-OSAS负载的GO的生物可及度(40.23%)显著高于Mix-GO(28.99%)和BSA-GO(23.16%)。研究结果证明BSA-OSAS制备的纳米粒子对GO有较好的保护效果,并可显著提高其生物可及度。The study aimed to improve the physicochemical property and bioavailability ofγ-oryzanol(GO).Glycosylated composite nanoparticles were prepared by a dry Maillard reaction of bovine serum albumin(BSA)-octenyl succinate anhydride starch(OSAS),and was used to encapsulate GO.When the mass ratio of BSA-OSAS glycosylated composite nanoparticles to GO was 40∶1,the particle size of BSA-OSAS-GO was 111.75 nm,and its encapsulation efficiency(84.46%)was significantly higher than that of the control groups(BSA-GO,62.78%;Mix-GO,75.67%).The BSA-OSAS-GO(55.44%)scavenging rate on the DPPH radical was significantly higher than that of BSA-GO(48.32%)and Mix-GO(41.03%).Compared with the control groups,BSA-OSAS-GO nanoparticles had better anti-digestion(the particle size was only increased by 262.26 nm)and protected GO more effectively(retention rate of GO was 84.45%after stored in 4℃for 15 days).After 180 min of simulated digestion in vitro,the bioavailability of BSA-OSAS-GO(40.23%)was significantly higher than that of Mix-GO(28.99%)and BSA-GO(23.16%).Results showed that the physicochemical property and bioavailability of GO could be improved by BSA-OSAS glycosylated composite nanoparticles.

关 键 词：糖基化 抗氧化性 贮藏稳定性 生物可及度 包埋 

分 类 号：TS201.2[轻工技术与工程—食品科学]