基于多重PCR定性鉴别鹿肉掺假  

Qualitative identification of adulterated venison based on multiplex PCR

作　　者：邵博宇 徐宁 迟凯月 于文莹 高丽君 艾金霞 罗雁非 李明成 SHAO Boyu;XU Ning;CHI Kaiyue;YU Wenying;GAO Lijun;AI Jinxia;LUO Yanfei;LI Mingcheng(Medical Technology School,Beihua University,Jilin 132013,China;Technical Center of Changchun Customs,Changchun 130062,China;Chinese Medicine DNA Fingerprint Detection Technology Innovation Center,Beihua University,Jilin 132013,China)

机构地区：[1]北华大学医学技术学院,吉林吉林132013 [2]长春海关技术中心,吉林长春130062 [3]北华大学吉林省中药DNA指纹检测技术科技创新中心,吉林吉林132013

出　　处：《食品与发酵工业》2022年第24期281-287,共7页Food and Fermentation Industries

基　　金：吉林省科技厅重点科技成果转化项目(20170307001YY);吉林省科技发展计划项目资助(20200404152YY;20200403047SF);北华大学研究生创新计划项目【2021】060号。

摘　　要：该研究建立鹿肉掺假快速鉴定方法,可对掺假肉的种类进行定性鉴定。建立一种应用多重聚合酶链式反应技术鉴别梅花鹿肉中牛肉、猪肉的掺假鉴定方法。提取梅花鹿肉、牛肉、猪肉3个物种肉组织的基因组DNA,通过多重PCR对梅花鹿、牛、猪的特异性片段进行扩增,鉴别样品中是否含有梅花鹿、牛、猪3种动物源性成分。通过对特异性片段的PCR扩增,可同时将梅花鹿、牛、猪的肉与其他物种进行区分。该研究建立了一种基于多重PCR技术快速、准确鉴别梅花鹿中掺假牛、猪等其他肉类的方法,为解决食用药用性产品易掺假、难以鉴定的问题提供了一个新途径。This study establishes a rapid identification method for venison adulteration,and the type of adulterated meat can be qualitatively identified.To establish a method of adulteration of beef and pork in plum venison using the multi-polymerase chain reaction technique.To extract the genomic DNA of the meat tissue of three species of plum venison,beef and pork,and to amplify the specific fragments of Cervus nippon,cattle and pigs through multiple PCR to identify whether the samples contained three animal-derived components of Cervus nippon,cattle and pigs.By amplifying PCR of specific fragments,the meat of Cervus nippon,cattle and pigs can be distinguished from other species at the same time.In this study,a rapid and accurate method based on multiplex PCR was developed to identify adulterated cattle,pigs and other meat in Cervus nippon,providing a new way to solve the problem of adulteration and difficulty in identifying edible medicinal products.The method was developed to provide a new way to solve the problem of adulteration of edible medicinal products.

关 键 词：多重PCR 种属鉴别 鹿肉 食品安全 成分检测 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]