响应面法优化桑椹多糖提取工艺及其抗氧化活性研究  

Optimization of extraction technology and antioxidant activity of Fructus mori polysaccharide by response surface method

作　　者：王振伟[1] WANG Zhen-wei

机构地区：[1]黄河水利职业技术学院,河南开封475004

出　　处：《饲料研究》2022年第21期87-92,共6页Feed Research

基　　金：河南省科技攻关项目(项目编号:202102310150);开封市重大科技专项(项目编号:2021029A);河南省高等学校青年骨干教师培养计划项目(项目编号:2018GGJS216)。

摘　　要：试验旨在优化桑椹多糖的提取工艺,研究桑椹多糖的抗氧化活性。试验分别考察温度、液料比、功率和时间对桑椹多糖得率的影响,通过DPPH·和OH·清除试验评估桑椹多糖的抗氧化活性。结果显示:桑椹多糖最优提取工艺是提取温度55℃、液料比20 mL/g、提取功率180 W、提取时间30 min。在所选条件下,桑椹多糖的实际提取得率为10.54%。桑葚多糖对DPPH·和OH·均具有较强的清除作用,其IC50值分别为0.376和0.244 g/L。研究表明,响应面优化桑椹多糖超声辅助提取工艺稳定、可行,且桑椹多糖具有较强的抗氧化活性。The experiment was to optimize the extraction process of Fructus mori polysaccharideie, studied its antioxidant activity. The effect of temperature, material to liquid ratio, power and time on yield of Fructus mori polysaccharide were investigated, respectively, and the antioxidant activity of Fructus mori polysaccharide was evaluated by DPPH·and OH·scavenging tests. The results showed that the optimum extraction parameters of Fructus mori polysaccharide were extraction temperature 55 ℃, liquid material ratio 20 mL/g, extraction power 180 W and extraction time 30 min. Under the conditions, the actual extraction rate of Fructus mori polysaccharide was 10.54%. Fructus mori polysaccharide has a strong scavenging effect on DPPH·and OH·, and its IC50was 0.376 and 0.244 g/L, respectively. The study indicates that the ultrasonic assisted extraction process of Fructus mori polysaccharide optimized by response surface methodology is stable and feasible, and Fructus mori polysaccharide has strong antioxidant activity.

关 键 词：桑椹 响应面法 超声辅助提取 多糖 抗氧化 

分 类 号：S816.7[农业科学—饲料科学]