丁香酚、香芹酚和百里香酚对禾谷镰刀菌的抑菌活性及机制  

Antibacterial Activity and Mechanism of Eugenol, Carvacrol and Thymol against Fusarium graminearum

作　　者：崔醒 朱秋劲 侯瑞[2] 万婧 CUI Xing;ZHU Qiujin;HOU Rui;WAN Jing(School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;College of Forestry,Guizhou University,Guiyang 550025,China;Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region,Ministry of Education,Key Laboratory of Animal Genetics,Breeding of Reproduction in the Plateau Mountainous Region,Ministry of Education,College of Life Sciences/Institute of Agro-Bioengineering,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州大学林学院,贵州贵阳550025 [3]贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与种质创新教育部重点实验室,高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025

出　　处：《食品科学》2022年第23期10-18,共9页Food Science

基　　金：国家自然科学基金地区科学基金项目(3216160258,32060554);贵州省科技厅一般基础项目(黔科合基础ZK[2021]176)。

摘　　要：以转录组技术为基础,研究丁香酚、香芹酚和百里香酚对禾谷镰刀菌的抑制作用及作用机理。方法:借助体外抑菌实验测定丁香酚、香芹酚和百里香酚对禾谷镰刀菌菌丝生长、孢子萌发和呕吐毒素生物合成的有效作用浓度;通过测定丁香酚、香芹酚和百里香酚处理后菌丝液的电导率、ATP含量和丙二醛含量进一步从细胞水平探讨其作用位点;通过转录组学阐明丁香酚、香芹酚和百里香酚抑制禾谷镰刀菌生长和毒素合成的分子机制。结果:3种植物精油活性成分中,百里香酚的抗真菌活性最强。丁香酚、香芹酚和百里香酚可破坏禾谷镰刀菌细胞膜完整性,引起电解质渗漏,能量代谢失衡。此外,3种植物精油活性成分通过核糖体、线粒体结构和转录组水平上的酶合成发挥对禾谷镰刀菌的抑制作用。The inhibitory effects and mechanism of eugenol, carvacrol and thymol against Fusarium graminearum were studied by transcriptomics. The effective concentrations of eugenol, carvacrol and thymol against the mycelium growth,spore germination, and deoxynivalenol biosynthesis of F. graminearum were determined through in vitro antifungal tests.At the cellular level, the action sites were further explored by measuring the conductivity, adenosine triphosphate(ATP)level and malondialdehyde(MDA) level of the mycelium solution treated with each of the three compounds. Thymol had the best antifungal activity among the three compounds. All these compounds could damage the cell membrane integrity of F. graminearum, causing electrolyte leakage and an imbalance in energy metabolism. In addition, they could exert antifungal activity against F. graminearum through regulating the ribosome and mitochondrial structure and enzyme synthesis at the transcriptome level.

关 键 词：丁香酚 香芹酚 百里香酚 禾谷镰刀菌 转录组 

分 类 号：TS201.6[轻工技术与工程—食品科学]