岩藻黄素改善视网膜色素上皮细胞吞噬功能的非抗氧化途径  

Non-Antioxidant Pathway for the Ameliorative Effect of Fucoxanthin against Phagocytic Disturbance of Retinal Pigment Epithelial Cells

作　　者：郭紫欣 刘芸君 刘翼翔[1] 王彦波 GUO Zixin;LIU Yunjun;LIU Yixiang;WANG Yanbo(College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China;School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China)

机构地区：[1]集美大学海洋食品与生物工程学院,福建厦门361021 [2]浙江工商大学食品与生物工程学院,浙江杭州310018

出　　处：《食品科学》2022年第23期168-173,共6页Food Science

基　　金：福建省杰出青年科学基金项目(2020J06024)。

摘　　要：本研究通过模拟视网膜高聚光、高多不饱和脂肪酸的生理环境,以分化的视网膜色素上皮(retinal pigment epithelium,RPE)细胞单层为体外评价模型,研究岩藻黄素对RPE细胞吞噬功能的改善作用及其非抗氧化作用途径。结果表明,当RPE细胞暴露于富含二十二碳六烯酸的可见光环境(3 500 lx),其吞噬功能明显降低,并且细胞膜上的Mer酪氨酸激酶(Mer tyrosine kinase, MerTK)、αV整合素蛋白、β5整合素蛋白及CD36吞噬受体的表达受到了不同程度的抑制。岩藻黄素(质量浓度10.0、20.0μg/m L)预处理能显著改善RPE细胞的吞噬功能并上调上述吞噬受体的表达。特异性抗体实验进一步证实,岩藻黄素主要通过调节Mer TK、αV整合素蛋白和β5整合素蛋白受体表达改善RPE细胞的吞噬功能。L-型Ca^(2+)通道也被证实是岩藻黄素改善RPE细胞吞噬功能的重要途径,这为利用岩藻黄素开发视力保护产品提供了依据。In this work, the ameliorative effect of fucoxanthin on visible light-induced phagocytic disturbance of differentiated retinal pigment epithelial(RPE) cells was investigated in a polyunsaturated fatty acids-rich environment and the non-antioxidant pathway involved was explored. The results showed that when exposed to visible light at 3 500 lx,the phagocytic capacity of RPE cells was significantly reduced, and the expression of phagocytic receptors(including Mer tyrosine kinase(MerTK), αV integrin protein, β5 integrin protein and CD36 receptor) on the cell membrane was inhibited to varying degrees. Pretreatment with different concentrations(10.0 and 20.0 μg/mL) of fucoxanthin significantly ameliorated the phagocytic capacity of RPE cells and effectively up-regulated the expression of the above four phagocytic receptors. Specific antibody testing further confirmed that fucoxanthin enhanced the phagocytic capacity of RPE cells mainly by regulating the expression of MerTK, αV integrin protein and β5 integrin protein receptors.In addition, the L-type Ca^(2+)channel was also shown to be an important pathway for fucoxanthin to improve the phagocytic capacity of RPE cells. These findings provide a theoretical basis for the development of vision-protective functional foods containing fucoxanthin.

关 键 词：岩藻黄素 视网膜色素上皮细胞 吞噬作用 吞噬受体 可见光 

分 类 号：TS201.6[轻工技术与工程—食品科学]