黑果腺肋花楸汁对便秘小鼠肠道功能的改善作用  

Ameliorating Effect of Aronia Melanocarpa Juice on Intestinal Function in Constipated Mice

作　　者：唐晓姝 陈雪梅[1,2] 胡博 张白曦 Tang Xiaoshu;Chen Xuemei;Hu Bo;Zhang Baixi(National Engineering Research Center for Functional Food 2School of Food Science and Technology,Jiangnan University,Wuxi 214000)

机构地区：[1]江南大学国家功能食品工程技术研究中心,江苏无锡214000 [2]江南大学食品学院,江苏无锡214000

出　　处：《中国食品学报》2022年第12期125-133,共9页Journal of Chinese Institute Of Food Science and Technology

摘　　要：为研究黑果腺肋花楸汁改善小鼠便秘,维护肠道健康的作用,将小鼠分为5组:空白对照组、便秘模型对照组、黑果腺肋花楸汁低、中、高剂量组,用样品干预14 d后通过盐酸洛哌丁胺造便秘模型,测定各组小鼠的排便功能、肠道运动、胃肠调节肽指标和16S rRNA肠道菌群指标。结果显示:便秘模型成立,黑果腺肋花楸汁可以缩短首粒黑便排出时间,增加5 h内排便粒数和质量,提高小肠墨汁推进率,促进胃泌素(Gas)、胃动素(MTL)和P物质(SP)含量,降低生长抑素(SS)、内皮素(ET-1)和血管活性肠肽(VIP)含量,且具有一定的剂量相关性。此外,黑果腺肋花楸汁在一定程度上可改变肠道菌群的分布,丰富物种的丰度,显著降低厚壁菌门与拟杆菌门相对丰度比值(F/B),降低便秘小鼠变形菌门、葡萄球菌属的丰度。研究表明黑果腺肋花楸汁对盐酸罗哌丁胺诱导的便秘小鼠有通便作用,其机制是通过调节肠道激素的分泌和肠道菌群结构来改善便秘症状。This paper aims to explore the effect of aroniamelanocarpa juice on improving constipation and maintaining intestinal health in mice.The mice were divided into five groups:blank control group,constipation model control group and low,medium and high dose group of aroniamelanocarpa juice.After 14 days of sample intervention,the constipation model was established by loperamide hydrochloride.The defecation function index,intestinal motility index,gastrointestinal regulatory peptide index and 16S rRNA intestinal flora index of mice in each group were measured.The results showed that the constipation model was established.Aroniamelanocarpa juice could shorten the discharge time of the first black stool,increase the number and quality of defecation granules within 5 hours,improve the propulsion rate of small intestinal ink,promote the contents of gastrin(Gas),motilin(MTL)and substance P(SP),reduce the contents of somatostatin(SS),endothelin(ET-1)and vasoactive intestinal peptide(VIP),the effect has a certain dose correlation.In addition,aroniamelanocarpa juice can change the distribution of intestinal flora and enrich the abundance of species to a certain extent,significantly reduce the relative abundance ratio of Firmicutes and Bacteroidota(F/B),reduce the abundance of Proteobacteria and Staphylococcus in constipated mice.Therefore,the study provides that aronia melanocarpa juice had a defecation effect on mice with constipation induced by loperamide hydrochloride,and its mechanism may be to improve the symptoms of constipation by regulating the secretion of intestinal hormones and the structure of intestinal flora.

关 键 词：黑果腺肋花楸汁 润肠通便 胃肠激素 肠道菌群 

分 类 号：R574.62[医药卫生—消化系统] TS201.4[医药卫生—内科学]