10-羟基-12-十八碳烯酸的静息细胞合成  

Resting Cell Biosynthesis of 10-Hydroxy-12-octadecenoic Acid

作　　者：郭前婉 彭舒悦 王晨晨 赵萌[1] Michael G.Gaenzle Guo Qianwan;Peng Shuyue;Wang Chenchen;Zhao Meng;Michael GGaenzle(Key Laboratory of Fermentation Engineering Ministry of Education,Hubei Key Laboratory of Industrial Microbiology,Hubei University of Technology,Wuhan 4300682Department of Agricultural,Food and Nutritional Science,University of Alberta,Edmonton,T6G 2P5,Canada)

机构地区：[1]发酵工程教育部重点实验室,湖北省工业微生物重点实验室湖北工业大学,武汉430068 [2]阿尔伯塔大学农业、食品与营养科学系,加拿大埃德蒙顿T6G 2P5

出　　处：《中国食品学报》2022年第12期144-152,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31972203);湖北省科技厅国际合作项目(DWHZ2017000010)。

摘　　要：10-羟基-12-十八碳烯酸(10-HOE)是一种典型的微生物源羟基不饱和脂肪酸,具有抑菌、抗炎、抗过敏等功效,在食品、药品、化工等行业具有很好的应用前景,因此,10-HOE的生物合成十分重要。本文以亚油酸为底物,以含10-亚油酸水合酶的重组大肠杆菌为催化剂,采用静息细胞法生物合成10-HOE,通过优化黄素腺嘌呤二核苷酸(FAD)浓度、溶剂种类及含量、甘油含量、氯化钠浓度、转化温度、溶液pH值、菌浓度、底物浓度和培养时间等9个单因素转化实验,来提高10-HOE的转化率。细胞转化生物合成的优化条件为:FAD浓度0.25 mmol/L,添加2%的正己烷,氯化钠浓度15 mmol/L,亚油酸底物质量浓度20 g/L,菌质量浓度200 g/L、溶液pH7.0,在37℃、200 r/min的摇床中培养24 h,获得的10-HOE转化率为(73.1±0.9)%,转化质量浓度为(16.3±0.2)g/L。该转化是目前为止10-HOE生物合成报道的最高转化浓度。本研究为大规模制备10-HOE提供了工艺路线,为工业化生产10-HOE提供参考。10-Hydroxy-12-octadecenoic acid(10-HOE),one microbial hydroxy unsaturated fatty acid,was recently reported to have the bioactivities of the anti-fungi,anti-inflammatory,and anti-allergic effects.It has the potential to be used in food,pharmaceutical,chemical,and other industries.Thus,the biosynthesis of 10-HOE is worthy of investigation.In this work,10-HOE was biosynthesized using the resting cell system with linoleic acid as the substrate and the recombinant Escherichia coli as the catalyst.After the single-factor experiment,the optimum conditions for the 10-HOE biosynthesis using resting cells were obtained,eg.0.25 mmol/L flavin adenine dinucleotide(FAD),2%hexane,15 mmol/L sodium chloride,20 g/L linoleic acid,and 200 g/L cells at pH 7.0.The mixture was incubated at 37°C and 200 r/min after a thorough mixing with a vortex mixer,and a conversion yield of(73.1±0.9)%with the 10-HOE concentration of(16.3±0.2)g/L was achieved at 24 h.Within our knowledge,this is the highest concentration for the10-HOE biosynthesis.This work provided one method of the 10-HOE biosynthesis,which might be helpful for the industrial production.

关 键 词：10-羟基-12-十八碳烯酸(10-HOE) 生物合成 静息细胞 试验优化 单因素实验 

分 类 号：TQ921[轻工技术与工程—发酵工程]