高产乙酸乙酯毕赤酵母筛选及其耐受性能研究  

Screening of Yeast Strain with High Yield of Ethyl Acetate and Study on Its Tolerance Characteristics

作　　者：李群 林斌 柯锋 乐细选 杨强 杨生智 陈申习 LI Qun;LIN Bin;KE Feng;YUE Xixuan;YANG Qiang;YANG Shengzhi;CHEN Shenxi(Hubei Key Lab for Quality and Safety Control of Traditional Chinese Medicine and Healthcare Food,Jing Brand Research Institute,Jing Brand Co.Ltd.,Daye,Hubei 435100,China)

机构地区：[1]劲牌有限公司,劲牌研究院,中药保健食品质量与安全湖北省重点实验室,湖北大冶435100

出　　处：《酿酒科技》2022年第12期39-45,共7页Liquor-Making Science & Technology

基　　金：湖北省重点研发计划项目(2020BBA050)。

摘　　要：为筛选高产乙酸乙酯酵母,对公司菌种库保藏的45株毕赤酵母进行了初筛和复筛,获得5株产酯效果较好的菌株Y10、Y87、Y14、Y42和J32。费比恩毕赤酵母(Pichia fabianii)Y10在实验室固态发酵条件下产乙酸乙酯含量最高,达到1.21 g/L,相比对照组(桂花曲)提升57.1%;将Y10扩大培养制成麸皮曲,应用于清香型小曲白酒中试车间,生产原酒中乙酸乙酯含量达到1.08 g/L,相较于桂花曲提高13.7%,且出酒率相当;对Y10进行耐受性实验,结果显示该菌株能分别耐受500 g/L葡萄糖、6%乙醇、pH3.0、37℃和8%NaCl条件,说明Y10能在复杂的白酒酿造环境中生长,为大车间进一步应用提供了指导。In order to obtain yeast strains with high yield of ethyl acetate,45 Pichia strains preserved in the company’s culture center were screened,and five strains with relatively high yield of ethyl acetate were obtained(Y10,Y87,Y14,Y42,J32).Among them,strain Pichia fabianii Y10 had the highest yield of ethyl acetate(1.21 g/L)under laboratory conditions,which was 57.1%higher than that of the control group.Then Y10 was used to make Fuqu,which was used to produce Xiaoqu Qingxiang Baijiu in a pilot-scale workshop.The content of ethyl acetate in the crude liquor reached up to 1.08 g/L,13.7%higher than the control,while the liquor yield remained stable.Environment tolerance tests were carried out on Y10,and the results showed that it could tolerate 500 g/L sugar,6%ethanol,pH 3.0,37℃,and 8%NaCl,respectively,indicating that Y10 could grow in the complex Baijiu fermentation environment.This study has provided guidance for further application of the strain in industrial-scale production.

关 键 词：清香型小曲白酒 费比恩毕赤酵母 乙酸乙酯 酿酒生产 环境耐受性 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.1[轻工技术与工程—食品科学与工程]