不同机械伤处理对香蕉果皮活性氧代谢的影响  

Effects of different mechanical injury treatments on the metabolism of reactive oxygen species in banana peel

作　　者：王敏 殷菲胧 梁园丽 刘云芬 廖玲燕 潘中田[1] 帅良 Wang Min;Yin Feilong;Liang Yuanli;Liu Yunfen;Liao Lingyan;Pan Zhongtian;Shuai Liang(School of Food and Biological Engineering,Guangxi Key Laboratory of Health Care Food Science and Technology,Hezhou University,Hezhou 542899,China;College of Food Engineering,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]贺州学院食品与生物工程学院/广西康养食品科学与技术重点实验室,贺州542899 [2]大连工业大学食品学院,大连116034

出　　处：《农业工程学报》2022年第16期284-292,共9页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金项目(31860457);广西自然科学基金项目(2020GXNSFAA297153);广西高校中青年教师科研基础能力提升项目(2021KY0705)。

摘　　要：为探究不同机械伤处理对香蕉果皮活性氧代谢的影响,该研究以“桂蕉6号”为材料,采用划伤、穿刺、坠落3种不同机械伤处理,以未处理组为对照,测定贮藏过程中香蕉果皮活性氧代谢的变化,并进行相关性分析和主成分分析。结果表明,3种不同机械损伤处理均加速了香蕉果实硬度和叶绿素含量的下降;引起O_(2)-产生速率和H_(2)O_(2)含量的积累,其中划伤处理组的O_(2)-产生速率在贮藏第8天为对照组的2.0倍,H_(2)O_(2)含量为对照组的2.5倍,这可能与机械伤处理抑制了果皮组织中过氧化物酶、超氧化物歧化酶、过氧化氢酶及抗坏血酸过氧化物酶活性有关;同时,3种不同机械损伤处理加速细胞膜脂过氧化的进程,造成香蕉细胞膜透性及丙二醛含量的升高,其中对照、划伤、穿刺和坠落处理组的丙二醛含量在贮藏第8天分别是贮藏初期的3.3、3.4、5.4和7.5倍;此外,划伤和穿刺处理均可显著提高香蕉果皮中多酚氧化酶(Polyphenol Oxidase,PPO)活性(P<0.01)。相关性聚类热图分析表明,H_(2)O_(2)含量与香蕉果实硬度、叶绿素含量和抗坏血酸过氧化物酶活性呈极显著负相关(P<0.01),与超氧化物歧化酶活性呈显著负相关(P<0.05);同时,多酚氧化酶活性与H_(2)O_(2)含量、丙二醛含量和细胞膜透性呈现极显著正相关(P<0.05)。结合主成分分析可知,不同机械伤处理均可加速香蕉果皮活性氧的积累进而导致香蕉果实的衰老腐败,其中划伤处理对香蕉果皮活性氧代谢影响最大,其次是穿刺处理,坠落处理对香蕉果皮活性氧代谢影响最小。该研究阐明了香蕉果实机械伤、活性氧和贮藏品质三者的关系,并为香蕉采后不同机械伤控制提供理论依据。During harvesting and transportation,bananas are highly susceptible to mechanical damage,which will not only affect the aesthetics,but also act as a pathogen center to further infect normal fruits,leading to secondary infection and causing great losses to the storage and transportation of fruits.At present,the research on mechanical damage of banana mainly focuses on controlling the occurrence of mechanical damage,but there are fewer reports from the mechanism level.Therefore,in this experiment,three different mechanical damage treatments,including scratch,puncture and fall,were used,with untreated as the control group,and stored at(25±(1)°C and(80±(5)%RH for 8 d,with samples taken at 2 d intervals.Fruit hardness,chlorophyll content,cell membrane permeability,malondialdehyde content,reactive oxygen species content and antioxidant enzyme activities were measured to analyze the relationship between mechanical damage,reactive oxygen species and storage quality and their possible mechanisms.The results showed that the three different mechanical damage treatments accelerated the decrease of banana fruit hardness and chlorophyll content compared with the control;it inhibited the activities of Peroxidase(POD),Superoxide Dismutase(SOD),Catalase(CAT)and Ascorbate Peroxidase(APX)in banana peel,led to the accumulation of reactive oxygen species O_(2)-and H_(2)O_(2),accelerated the process of cell membrane lipid peroxidation,increased the permeability of banana cell membrane and Malondialdehyde(MDA)content,and then accelerated the deterioration of banana storage quality.The correlation clustering heat map analysis showed that O_(2)-production rate,H_(2)O_(2)content,MDA content and cell membrane permeability indexes were negatively correlated with each other and hardness,chlorophyll content,SOD activity and APX activity indexes.Among them,H_(2)O_(2)content was negatively correlated with hardness,chlorophyll content and APX activity(P<0.01)and with SOD activity(P<0.05),which was consistent with the gradual decrease of stora

关 键 词：储藏 主成分分析 香蕉 机械损伤 活性氧代谢 相关性分析 

分 类 号：S635.3[农业科学—蔬菜学]