Yajieshaba prevents lipopolysaccharide-induced intestinal barrier injury via anti-inflammatory and anti-apoptosis  

作　　者：YANG Liping YU Xinglin ZHANG Chao CHEN Pu DUAN Xiaohua() 

机构地区：[1]Yunnan Key Laboratory of Dai and Yi Medicines,Yunnan University of Chinese Medicine,Kunming 650500,China

出　　处：《Journal of Traditional Chinese Medicine》2022年第5期707-714,共8页中医杂志（英文版）

基　　金：Supported by Grant from the Scientific Research Project of Yunnan Provincial Hospital of Traditional Chinese Medicine:Protective Effect and Mechanism of Yajie Prescription on Intestinal Mucosal Barrier (No. 2015DYYJ101);Yunnan Provincial Science and Technology Department-Applied Basic Research Joint Special Funds of Yunnan University of Traditional Chinese Medicine:Study on the Protective Effect and Mechanism of the Dai Medicine"Yajieshaba"on Intestinal Mucosal Barrier in Food Allergic Mice (No. 2017FF116-026)。

摘　　要：OBJECTIVE: To investigate the protective effect of Yajieshaba(YJSB) on the intestinal barrier dysfunction induced by lipopolysaccharide(LPS).METHODS: C57BL/6 mice and rat intestinal epithelial cells were treated with LPS. Thiazolyl Blue Tetrazolium Bromide assay were used to detect cell viability. DLactate, diamine oxidase and myeloperoxidase and cytokines were determined by enzyme-linked immunosorbent assay. Western blot was used to detect apoptosis-related proteins and tight junction(TJ) proteins. Real-time quantitative polymerase chain reaction was used to quantify the levels of m RNA expression of cytokines. Histological analysis was performed by hematoxylin and eosin staining. An immunofluorescence staining assay was performed to determine the expression level of TJ protein. RESULTS: YJSB increased cell viability and decreased apoptosis, maintained intestinal permeability after LPSinduced. YJSB inhibited LPS-induced decrease of TJ protein expression, pro-inflammatory cytokine levels and neutrophil infiltration. CONCLUSION: YJSB protect against LPS-induced intestinal barrier dysfunction via anti-inflammatory and anti-apoptosis, suggesting its therapeutic potential against intestinal barrier injury-related diseases.

关 键 词：INFLAMMATION apoptosis LIPOPOLYSACCHARIDES Yajieshaba intestinal barrier function 

分 类 号：R285.5[医药卫生—中药学]