Zebrafish facilitates drug screening: potential of 3-deoxy-andrographoside from Chuanxinlian(Herba Andrographitis Paniculatae) as an anti-inflammatory agent  

作　　者：HE Xuemei XIAO Junjie FAN Chunlin LU Zibin CAO Huihui YU Linzhong ZHENG Yuanru LIU Junshan 

机构地区：[1]Third Level Research Laboratory of State Administration of Traditional Chinese Medicine,School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China [2]Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics,Guangzhou 510515,China [3]Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research,College of Pharmacy,Jinan University,Guangzhou 510632,China [4]School of Pharmacy,Guangdong Pharmaceutical university,Guangzhou 510006,China [5]Department of Pharmacy,Zhujiang Hospital,Southern Medical University,Guangzhou 510515,China

出　　处：《Journal of Traditional Chinese Medicine》2022年第5期749-757,共9页中医杂志（英文版）

基　　金：Supported by the National Science Foundation-funding Project:Study of the Attenuating Effects of 3-dehydroandrographolide on Acute Lung Injury through Modulating Alveolar Macrophage Polarization via Targeting α7n ACh R (No. 81973544);the Guangzhou Education Bureau University Scientific Research Project:Construction of Traditional Chinese Medicine Discipline (no. 201831845);the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme:Study on the Mechanisms of Anti-Tumor and AntiInflammatory Components from Traditional Chinese Medicine based on Detoxification Therapy (No. GDHVPS2018);the Young Elite Scientists Sponsorship Program by CACM:Study on the Mechanisms of Antitumor Components from Traditional Chinese Medicine based on Detoxification Therapy (No. 2019-QNRC2-C14)。

摘　　要：OBJECTIVE: To systematically evaluate the in vivo antiinflammatory potential of diterpene lactones from Chuanxinlian(Herba Andrographitis Paniculatae)(AP). METHODS: We firstly adopted zebrafish, a novel and ideal animal model for high-throughput drug screening, to investigate the in vivo anti-inflammatory activities of 17 diterpene lactones isolated from AP.RESULTS: The results showed that most of diterpene lactones displayed significant anti-inflammatory effects in lipopolysaccharide microinjection-, copper sulfate exposure-or tail transection-induced zebrafish inflammation models. Moreover, diterpene lactone 3-deoxy-andrographoside(AP-5) was firstly found to attenuate inflammatory responses, which was closely associated with the myeloid differentiation primary response 88/nuclear factor-kappa B and signal transducer and activator of transcription 3 pathways. CONCLUSION: Our research sheds light on the inestimable roles of zebrafish in high-throughput drug screening, elucidates the potent inhibitory effects of diterpene lactones against inflammation and indicates that AP-5 may serve as a potential alternative agent for the treatment of inflammatory diseases.

关 键 词：diterpenes lactones INFLAMMATION ZEBRAFISH myeloid differentiation factor 88 NF-kappa B STAT3 transcription factor 

分 类 号：R285.5[医药卫生—中药学]