马铃薯资源糖苷生物碱含量评价及合成相关基因表达分析  

Evaluation of Steroidal Glycoalkaloids and Analysis of Related Gene Expression in Potato Resources

作　　者：叶广继[1,2] 郑贞贞[1] 纳添仓[1,2] 王舰 YE Guangji;ZHENG Zhenzhen;NA Tiancang;WANG Jian(Qinghai Academy of Agriculture and Forestry Sciences,Xining 810016,China;Qinghai Provincial Key Laboratory of Potato Breeding,Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources,Qinghai University Key Laboratory of Qinghai-Tibet Plateau Biotechnology of the Ministry of Education,Provincial and Ministry Co-construction of the State Key Laboratory of Sanjiangyuan Ecology and Plateau Agriculture and Animal Husbandry,Xining 810016,China)

机构地区：[1]青海省农林科学院,西宁810016 [2]青海省马铃薯育种重点实验室,青藏高原种质资源研究与利用实验室,青海大学青藏高原生物技术教育部重点实验室,省部共建三江源生态与高原农牧业国家重点实验室,西宁810016

出　　处：《园艺学报》2022年第11期2357-2366,共10页Acta Horticulturae Sinica

基　　金：三江源生态与高原农牧业国家重点实验室开放课题(2014-08);青海省自然科学基础研究项目(2022-ZJ-902);青海省创新平台建设项目(2022-ZJ-Y01)。

摘　　要：检测11个马铃薯资源叶片和薯皮中糖苷生物碱(Steroidalglycoalkaloids,SGA)含量,筛选低SGA含量的马铃薯资源,并对下寨65、LZ111、Q072625、陇薯7号、青薯9号和青薯11号薯皮中SGA合成途径中相关基因的表达进行分析,以期明确马铃薯SGA生物合成关键基因,为创制低SGA马铃薯资源提供新思路。结果表明:(1)11个马铃薯资源叶片中SGA含量范围为0.08600~1.58066 mg·g^(-1)FW;(2)11个马铃薯资源薯皮中SGA含量范围为0.01415~0.18450 mg·g^(-1)FW,薯皮总SGA含量均小于0.20 mg·g^(-1)FW(安全范围);(3)6个马铃薯资源块茎中SGA合成途径相关基因表达量分析表明,StSQS1、StCAS、StSSR2和StGAME4是马铃薯块茎SGA生物合成的关键基因。The content of SGAs(Steroidal glycoalkaloids)was evaluated in the leaf and peel tissue of 11 potato resources,the resources with relatively low level of SGAs were selected.The gene expression related to the SGAs biosynthesis was determined in Xiazhai 65,LZ111,Q072625,Longshu 7,Qingshu 9,and Qingshu 11,which would help confirm the key genes in SGAs biosynthesis of potato and provide the potential to create the low SGAs potato resources in the future.These results showed that SGAs content in the leaves of 11 cultivars was 0.08600–1.58066 mg·g^(-1)FW;the SGAs content in the peel tissue of potato tubers was 0.01415–0.18450 mg·g^(-1)FW and lower than 0.20 mg·g^(-1)FW(the safety level).The genes of StSQS1,StCAS,StSSR2 and StGAME4 played key roles in SGAs biosynthesis.

关 键 词：马铃薯 糖苷生物碱 基因表达 

分 类 号：S532[农业科学—作物学]