桑椹果粉乙醇提取液对秀丽隐杆线虫衰老的影响及机制  

Effects and Mechanism of Ethanol Extract of Mulberry Fruit Powder on Aging of Caenorhabditis elegans

作　　者：曾智康 朱晨 魏瑞霖 段树华 安淦鼎 孙在兴 雷激 李玉锋 ZENG Zhikang;ZHU Chen;WEI Ruilin;DUAN Shuhua;AN Ganding;SUN Zaixing;LEI Ji;LI Yufeng(School of Food and Biological Engineering,Xihua University,Chengdu 610036,China)

机构地区：[1]西华大学食品与生物工程学院,四川成都610036

出　　处：《食品工业科技》2023年第3期414-420,共7页Science and Technology of Food Industry

基　　金：四川省科技厅重大项目四川特色水果加工关键技术研究及产品开发(2020YFN0149)。

摘　　要：目的:探究桑椹果粉乙醇提取液对秀丽隐杆线虫动物模型(Caenorhabditis elegans,C.elegans,线虫)抗衰老的作用机制。方法:研究桑椹果粉乙醇粗提取液(Mulberry ethanol extract,MEE)对线虫刺激后,其寿命、抗热应激、运动能力、吞咽能力、生殖能力的变化,同时进行了线虫体的SOD活力、CAT活力及抗衰老基因转录水平等研究。结果:与对照组相比,用5 mg/mL的MEE刺激线虫后使剂量组最长寿命极显著增加了22.24%(P<0.01)、显著提高了线虫运动能力和抗热应激能力(P<0.05),使吞咽能力提升了30.43%且不显著影响线虫的生殖能力(P>0.05),同时在该刺激下线虫体内的SOD、CAT酶活力分别增加了21.44和13.84 U/mg prot,氧化应激反应阈值降低。经5 mg/mL的MEE处理后,抗氧化基因sir-2.1、skn-1表达水平分别上调了45.70%和51.73%,胰岛素基因daf-16表达水平上调161.63%、daf-2表达水平下调57.10%,寿命和抗压力、热冲击性能的关键调节基因hsp-16.2、hsf-1表达水平分别上调了121.13%和41.07%。结论:桑椹果粉乙醇提取液通过增强线虫体内SOD、CAT的活力,上调体内各组基因的表达水平达到抗衰老的作用。Objective:To investigate the mechanism of anti-aging effect of ethanolic extract of mulberry fruit powder on the animal model of Caenorhabditis elegans(C.elegans,nematode).Methods:To study the changes in longevity,resistance to heat stress,motility,swallowing ability and reproductive ability of nematodes after stimulation with mulberry ethanol extract(MEE).And SOD activity,CAT activity and transcript levels of anti-aging genes in nematode bodies were also investigated.Results:When the nematodes were stimulated with 5 mg/mL of MEE,the maximum lifespan extremely significantly increased by 22.24%compared to the control group(P<0.01),significantly improved the nematode motility and resistance to heat stress(P<0.05),and did not affect the reproductive capacity of the nematodes(P>0.05)when the swallowing capacity was improved by 30.43%compared to the control group.Meanwhile,the results showed that the enzymatic activity of SOD and CAT enzymes in nematodes increased by 21.44 and 13.84 U/mg prot,respectively,reducing the threshold of oxidative stress response.After treatment with 5 mg/mL of MEE,the expression levels of antioxidant genes sir-2.1 and skn-1 were up-regulated by 45.70%and 51.73%,respectively,and the expression levels of insulin genes daf-16 were up-regulated by 161.63%and daf-2 were down-regulated by 57.10%.The expression levels of hsp-16.2 and hsf-1,key regulators of life span and resistance to stress and thermal shock,were up-regulated by 121.13%and 41.07%.Conclusion:The ethanolic extract of mulberry fruit powder achieved anti-aging effects by enhancing the activity of SOD and CAT in nematodes and up-regulating the expression levels of various groups of genes in vivo.

关 键 词：秀丽隐杆线虫 抗衰老 桑椹果粉乙醇提取物 寿命 

分 类 号：TS201.4[轻工技术与工程—食品科学]