Microglia and astrocytes mediate synapse engulfment in a MER tyrosine kinase-dependent manner after traumatic brain injury  

作　　者：Hui Shen Xiao-Jing Shi Lin Qi Cheng Wang Muyassar Mamtilahun Zhi-Jun Zhang Won-Suk Chung Guo-Yuan Yang Yao-Hui Tang 

机构地区：[1]Med-X Research Institute and School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai,China [2]Department of Biological Sciences,Korea Advanced Institute of Science and Technology,Daejeon,South Korea

出　　处：《Neural Regeneration Research》2023年第8期1770-1776,共7页中国神经再生研究（英文版）

基　　金：supported by the National Key R&D Program of China,No.2019YFA0112000(to YHT);the National Natural Science Foundation of China,Nos.82071284(to YHT),81974179(to ZJZ);Shanghai Rising-Star Program,No.21QA1405200(to YHT);the Scientific Research and Innovation Program of Shanghai Education Commission,No.2019-01-07-00-02-E00064(to GYY);Scientific and Technological Innovation Act Program of Shanghai Science and Technology Commission,No.20JC1411900(to GYY);the Notional Research Foundation of Korea,Nos.2020M3E5D9079912(to WSC),2021R1A2C3005704(to WSC),2022M3E5E8081188(to WSC);the Korea Health Technology R&D Project,No.HU20C0290(to WSC)。

摘　　要：Recent studies have shown that microglia/macrophages and astrocytes can mediate synaptic phagocytosis through the MER proto-oncokinase in developmental or stroke models,but it is unclear whether the same mechanism is also active in traumatic brain injury.In this study,we established a mouse model of traumatic brain injury and found that both microglia/macrophages and astrocytes phagocytosed synapses and expression of the MER proto-oncokinase increased 14 days after injury.Specific knockout of MER in microglia/macrophages or astrocytes markedly reduced injury volume and greatly improved neurobehavioral function.In addition,in both microglia/macrophages-specific and astrocytes-specific MER knock-out mice,the number of microglia/macrophage and astrocyte phagocytosing synapses was markedly decreased,and the total number of dendritic spines was increased.Our study suggested that MER proto-oncokinase expression in microglia/macrophages and astrocytes may play an important role in synaptic phagocytosis,and inhibiting this process could be a new strategy for treating traumatic brain injury.

关 键 词：animal model astrocyte dendritic spines LYSOSOME macrophage MER proto-oncokinase MICROGLIA neurologic function phagocytosis synapse engulfment traumatic brain injury 

分 类 号：R651.15[医药卫生—外科学]