缢蛏对潮间带原油污染的氧化应激及IBR评价  

Oxidative stress and IBR evaluation of Sinonovacula constricta on intertidal oil pollution

作　　者：徐青霞 潘玉英 杨婷婷 杨金生 张萌 陈帆 王滢赢 唐忠伟 XU Qing-xia;PAN Yu-ying;YANG Ting-ting;YANG Jin-sheng;ZHANG Meng;CHEN Fan;WANG Ying-ying;TANG Zhong-wei(College of Fisheries of Zhejiang Ocean University,Zhoushan 316022,China;Key Laboratory of Marine Fishery Equipment and Technology of Zhejiang,Zhoushan 316022,China;College of Petrochemical Engineering&Environment,Zhejiang Ocean University,Zhoushan 316022,China)

机构地区：[1]浙江海洋大学,水产学院,浙江舟山316022 [2]浙江省海洋渔业装备技术研究重点实验室,浙江舟山316022 [3]浙江海洋大学,石油化工与环境学院,浙江舟山316022

出　　处：《中国环境科学》2023年第1期328-340,共13页China Environmental Science

基　　金：浙江省自然科学基金资助项目(LY19D060003);浙江省大学生科技创新活动计划(新苗人才计划)项目(2021R411006);国家级大学生创新创业训练计划项目(202110340052);浙江省属高校基本科研业务费项目(2019J00023)。

摘　　要：通过研究不同浓度原油污染对缢蛏(Sinonovacula constricta)鳃和内脏团抗氧化酶活性、脂质过氧化及鳃结构的影响,结合综合生物标志物响应(IBR),探讨潮间带原油污染对生物的毒性效应.结果显示:在剂量-效应方面,2种组织超氧化物歧化酶(SOD)活性和内脏团中谷胱甘肽过氧化物酶(GPx)总体上表现为低浓度诱导、高浓度抑制效应;SOD诱导与过氧化氢酶(CAT)抑制同时出现,规律大致相反.在时间-效应上,SOD活性呈升高-降低-升高的趋势,CAT与GPx呈先降低后升高的趋势;谷胱甘肽硫转移酶(GST)在鳃中呈现先升高后降低的趋势,酶活性最高为371.663U/mgprot.暴露前期(6h)缢蛏2种组织中丙二醛(MDA)含量显著增加,鳃和内脏团中MDA含量最高值分别为5.030和10.705nmol/mgprot,后期逐渐平稳.IBR结果表明鳃中生物标志物对原油污染敏感度更高.原油暴露会使鳃丝结构发生变形或引起鳃丝脱离等现象.研究表明,缢蛏鳃更适宜作为潮间带原油暴露生物监测与评价的器官.To discuss the toxic effect which induced by intertidal crude oil pollution to organisms,antioxidant enzyme activities and lipid peroxidation in gills and visceral mass,the changes of gills structure as well as Integrated Biomarker Response(IBR)of Sinonovacula constricta exposed to different oil concentration were studied.The results showed that superoxide dismutase(SOD)activities in gills and visceral mass as well as glutathione peroxidase(GPx)activities in visceral mass appeared low concentration induction and high concentration inhibition effect in terms of dose-response.SOD induction and catalase(CAT)inhibition appeared at the same time while the rules were roughly opposite.In the time-effect,the SOD activities showed an increase-decrease-increase trend,while CAT and GPx activities showed the decrease-increase trend.The Glutathione S-transferase(GST)activities in gills showed an increase-decrease trend,the maximum value was 371.663U/mgprot.The malondialdehyde(MDA)content in the two tissues of Sinonvacula constricta increased significantly in the early stage of exposure(6h),the highest MDA content in gills and visceral mass were 5.030 and 10.705 nmol/mgprot,respectively,then gradually stabilized in the later stage.IBR results showed that biomarkers in gills were more sensitive to crude oil contamination.The crude oil exposure can deform the gills filament structure or cause their detachment.The results showed that gills of Sinonovacula constricta were more suitable as the organ for biological monitoring and evaluation of oil exposure in intertidal zone.

关 键 词：原油 缢蛏 抗氧化酶 IBR 组织损伤 

分 类 号：X174[环境科学与工程—环境科学]