基于雷笋壳的氮掺杂碳量子点的制备及对四环素的检测  

Preparation of nitrogen-doped carbon quantum dots based on bamboo shoots shells and detection of tetracycline

作　　者：刘梦茹 张亚男 张馨月 黄嘉杨 周杨洁 蒋洁 陈梓月 陈姝娟[1] LIU Meng-Ru;ZHANG Ya-Nan;ZHANG Xin-Yue;HUANG Jia-Yang;ZHOU Yang-Jie;JIANG Jie;Chen Zi-Yue;CHEN Shu-Juan(College of Food Science,Sichuan Agricultural University,Ya’an,625014,China)

机构地区：[1]四川农业大学食品学院,雅安625014

出　　处：《食品安全质量检测学报》2022年第24期8008-8016,共9页Journal of Food Safety and Quality

基　　金：成都市科技局项目(2022-YF-00487-SN);四川省大学生创新训练计划项目(20211062611)。

摘　　要：目的以农业废弃物雷笋壳为原料,制备氮元素掺杂的碳量子点(nitrogen-doped carbon quantum dots,N-CQDs),作为快速检测四环素的绿色新型荧光探针。方法N-CQDs由水热法制备而成,同时通过透射电镜、X射线衍射分析、傅立叶红外光谱分析、X射线光电子能谱和荧光分光光度计等对N-CQDs进行表征。结果制备的N-CQDs具有晶格结构的球形形态,平均粒径为5.16 nm,表面官能团丰富,具有良好的水溶性,在高浓度离子溶液和较宽pH范围内的N-CQDs具有高荧光强度和优异的稳定性。N-CQDs对四环素的检测具有较好的选择性和抗干扰性,在0.05~0.50μg/mL范围内呈现良好的线性关系,检出限为0.024μg/mL。鱼肉样品和牛奶样品中的四环素的加标回收率为91.95%~103.80%,相对标准偏差(relative standard deviation,RSD)小于等于5.37%。结论与传统的检测四环素的方法相比,本研究构建的绿色环保的荧光探针具有成本低、操作简单的优点,且具有良好的重现性和稳定性。Objective To prepare nitrogen-doped carbon quantum dots(N-CQDs)from agricultural waste Lei bamboo shoot shell as a new green fluorescent probe for rapid detection of tetracycline.Methods N-CQDs were prepared by hydrothermal method.Meanwhile,N-CQDs were characterized by transmission electron microscopy、X-ray diffraction analysis,fourier infrared spectroscopy,X-ray photoelectron spectroscopy and fluorescence spectrophotometer.Results The prepared N-CQDs had a spherical shape of lattice structure,with an average particle size of 5.16 nm,rich surface functional groups and good water solubility.N-CQDs had high fluorescence intensity and excellent stability in high concentration ionic solutions and a wide pH range.N-CQDs had good selectivity and anti-interference for the detection of tetracycline,showing a good linear relationship in the range of 0.05-0.50μg/mL,and the limit of detection was 0.024μg/mL.The recovery of tetracycline in fish and milk samples were 91.95%-103.80%,and the relative standard deviation(RSD)was less than or equal to 5.37%.Conclusion Compared with the traditional methods for detecting tetracycline,the green fluorescent probe constructed in this study has the advantages of low cost,simple operation,and good reproducibility and stability.

关 键 词：竹笋壳 氮掺杂碳量子点 四环素 荧光检测 

分 类 号：O657.3[理学—分析化学] TS207.3[理学—化学]