烟草黑胫病的病原鉴定及诱抗剂处理效果  

Pathogen identification of tobacco black shank and determination of indoor control effect of plant resistance inducers

作　　者：田所会 刘涛[2,3] 刘俊 金红岗 李枝桦 谢永辉 陈严平[2] Tian Suohui;Liu Tao;Liu Jun;Jin Honggang;Li Zhihua;Xie Yonghui;Chen Yanping(Guangnan County No.10 Middle School,Wenshan Yunnan 663300,China;College of Agriculture and Biotechnology,Yunnan Agricultural University,Kunming Yunnan 650201,China;National-Local Joint Engineering Research Center on Gemplasm Innovation&Utilization of Chinese Medicinal Materials in Southwest China,Yunnan Agricultural University,Kunming Yunnan 650201,China;Technology center of Hongyun Honghe Tobacco Group,Kunming Yunnan 650201,China;Kunming Tobacco Company of Yunnan Province,Kunming Yunnan 650201,China)

机构地区：[1]云南省文山州广南县第十中学校,云南广南663300 [2]云南农业大学农学与生物技术学院,云南昆明650201 [3]云南农业大学西南中药材种质创新与利用国家地方联合工程研究中心,云南昆明650201 [4]红云红河烟草集团技术中心,云南昆明650201 [5]云南省烟草公司昆明市公司,云南昆明650201

出　　处：《中国植保导刊》2022年第10期20-24,66,共6页China Plant Protection

基　　金：雪茄烟叶质量控制评价体系研究及应用(HYHH2021YL01);烟草黑胫病全生长周期防控微生物菌剂的研发与应用(2020530-000242026)。

摘　　要：2020年6月在云南省昆明市安宁市采集有黑胫病典型症状的发病烟株,对病原菌进行分离纯化,致病性检测,结合形态学与分子生物学进行鉴定;并以盆栽施药法研究硫胺素和乳酸两种诱抗剂对烟草黑胫病的防治效果。结果显示,采自安宁市的烟草黑胫病样本,病原菌为烟草疫霉;采用10 mmol/L的硫胺素和7 mmol/L的乳酸对烟株进行叶面喷雾与淋茎处理能有效促进烟草生长,并对黑胫病具有一定防效,分别为21.95%、14.32%。Tobacco plants with typical symptoms of black shank disease in Anning of Yunan were collected in June 2020,and the pathogen was isolated and purified. The results of the pathogenicity test and morphological and molecular biological identification suggest that the pathogen of tobacco black shank disease in the Anning tobacco planting base was Phytophthora nicotianae. The control effects of thiamine and lactic acid on P. nicotianae were studied by pot test. Leaf and stem spray with thiamine(10 mmol/L) and lactic acid(7 mmol/L) could effectively promote the growth of tobacco, and the control effect against tobacco black shank was 21.95% and 14.32%, respectively.

关 键 词：烟草 诱抗剂 硫胺素 乳酸 烟草黑胫病 防治效果 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]