正相–高效液相色谱法测定菜籽油中菜籽多酚Canolol的研究  

Determination of Canolol in Rapeseed Oil by Normal Phase High Performance Liquid Chromatography

作　　者：李昕阳 李贵友[1] 杨微[1] 杨军[1] LI Xin-Yang;LI Gui-You;YANG Wei;YANG Jun(Sichuan Grain and Oil Center Supervision and Inspection station,Chengdu,Sichuan 610041,China)

机构地区：[1]四川省粮油中心监测站,四川成都610041

出　　处：《粮油食品科技》2023年第1期158-163,共6页Science and Technology of Cereals,Oils and Foods

基　　金：四川省科技计划项目(2021YFN0014)。

摘　　要：对快速测定菜籽油中菜籽多酚Canolol的检测方法进行了研究,建立了正相-高效液相色谱法(NP-HPLC)测定菜籽多酚的检测方法。以正己烷∶异丙醇体积比96∶4为流动相,在流速为0.8 mL/min,柱温30℃,荧光检测器激发波长298 nm,发射波长325 nm的条件下,测定菜籽油中的菜籽多酚,其线性相关系数为0.999,加标回收率在96%~110%之间;仪器检出限为15 ng/mL,精密度优于3%。该方法具有操作简便、分析时间短、准确度高、环境污染小的特点。采用优化后的检测方法,对90个全国不同地区不同品种的油菜籽采用压榨工艺生产的菜籽油样品进行了测定,Canolol含量在190~2246 mg/kg之间;同时检测了从市面上随机采购的不同类型植物油,结果表明菜籽多酚Canolol为压榨工艺菜籽油中特有成分。A rapid method for the detection of rapeseed polyphenols(Canolol)in rapeseed oil was studied.A normal phase high performance liquid chromatography(NP-HPLC)method for the determination of Canolol was established.The Canolol in rapeseed oil were determined by using hexane to isopropanol(volume ratio 96∶4)as the mobile phase with the flow rate of 0.8 mL/min,column temperature of 30℃,fluorescence detector with excitation wavelength of 298 nm and emission wavelength of 325 nm.The linear correlation coefficient was above 0.999,and the recovery rate was between 96%and 110%.The detection limit of the instrument was 15 ng/mL,and the precision was better than 3%.The method has the advantages of simple operation,short analysis time,high accuracy and less environmental pollution.The optimized detection method was used to determine the rapeseed oil samples from 90 different varieties of rapeseed in different regions of China.The content of Canolol ranged from 190 mg/kg to 2246 mg/kg.At the same time,different types of vegetable oils randomly purchased from the market were tested.The results showed that Canolol was a unique component in rapeseed oil by processing of heated screw-pressing.

关 键 词：菜籽油 菜籽多酚Canolol 正相-高效液相色谱法 压榨工艺 

分 类 号：S565.4[农业科学—作物学]