显齿蛇葡萄转录组测序、注释与分析  

Sequencing,Annotation and Analysis of Transcriptome Ampelopsis grossedentata(Hand.-Mazz.)W. T. Wang

作　　者：徐友阳[2] 王德勤[2] 李静宇 梅瑜[1] 王继华[1] 蔡时可[1] XU Youyang;WANG Deqin;LI Jingyu;MEI Yu;WANG Jihua;CAI Shike(Guangdong Provincial Engineering&Technology Research Center for Conservationand Utilization of the Genuine Southern Medicinal Resources/Key Laboratory of CropGenetic Improvement of Guangdong Province/Crop Research Institute Guangdong Academyof Agriculture Sciences,Guangzhou,Guangdong510515,China;HutchisonWhampoa Guangzhou Baiyunshan Chinese Medicine Co.,Ltd.,Guangzhou,Guangdong 510640,China)

机构地区：[1]广东省道地南药资源保护与利用工程中心/广东省农作遗传改良重点实验室/广东省农业科学院作物研究所,广东广州510640 [2]广州白云山和记黄埔中药有限公司,广东广州510515

出　　处：《农业研究与应用》2022年第5期15-23,共9页Agricultural Research and Application

基　　金：汕尾市省科技专项资金项目(220126225850658);广州市科技计划项目(20212210006);广东省农业科学院汕尾分院科技合作专项(2022)。

摘　　要：本研究通过高通量转录组测序和生物信息学分析获得显齿蛇葡萄的转录表达数据,提取显齿蛇葡萄叶片和茎的总RNA(Ribonucleic Acid,核糖核酸)、建库、测序、组装,注释和比对等生物信息分析技术得到显齿蛇葡萄转录组的遗传信息。共检测到23,208,741个高质量的Reads,对数据进行Denovo组装后,获得43,603个Unigene,其中Unigene的N50长度为1580 bp,平均长度为1000.2 bp。在KOG、GO、KEGG、NR、SwissProt数据库中进行比对,得到注释的有25714个(58.97%)Unigene。其中25,801个Unigene在Nr数据库中得到注释,15,934个Unigene在KEGG数据库中得到注释,涉及135条代谢通路。在显齿蛇葡萄中我们共鉴定到75个Unigene涉及黄酮类生物合成,其中参与黄酮和黄酮醇生物合成的Unigene有4个,参与异黄酮生物合成的Unigene有13个。同时还检测到922个转录因子(Transcription factors,TFs)。采用MISA(Microsatellite identification tool)对组装的1000 bp以上的unigene分析发现,4771个Unigene包含5869个简单重复序列(Simple sequence repeats,SSRs),并使用Primer 3.0设计引物。利用高通量测序和生物信息学分析获得了显齿蛇葡萄的转录组信息特征,转录组数据为显齿蛇葡萄的重要功能基因鉴定,次生代谢通的挖掘奠定了研究基础。This study obtained transcription expression data through high-throughput transcriptome sequencing and bioinformatics analysis and the total ribonucleic acid(RNA)of Ampelopsis grossedentata(Hand.-Mazz.)W.T.Wang leaves and stems were extracted to get genetic information of A.grossedentata(Hand.-Mazz.)W.T.Wang transcriptome through library building,sequencing,assembling,annotating and alignment.Detected were 23208741 high-quality reads.A total of 43603 unigenes were obtained by de nove assembly,with an average N50 length of 1580 bp and an average length of 1,000.2 bp.25714(58.97%)unigenes were annotated in a KOG,GO,KEGG,NR and SwissPrott databases,among which 25801 unigenes were annotated in the NR database,15934 unigenes were annotated in the KEGG database,involving 135 metabolic pathways.In A.grossedentata(Hand.-Mazz.)W.T.Wang,75 unigenes were identified to be involved in flavonoid biosynthesis,4 unigenes involved in flavone and flavonol biosynthesis,and 13 unigenes involved in isoflavonoid biosynthesis.922 transcription factors(TFs)were detected.In addition,microsatellite identification tool(MISA)software was used to perform simple sequence repeats(SSRs)detection on the unigenes more than 1000bp,and a total of 5,869 SSR loci in 4771 unigenes were identified and primers were designed using Primer 3.0.Transcriptome information characteristics of A.grossedentata(Hand.-Mazz.)W.T.Wang were obtained by the high-throughput sequencing technology and bioinformatics analysis,and the transcriptome data provided a research basis for future research to identify functional genes and secondary metabolic pathways of A.grossedentata(Hand.-Mazz.)W.T.Wang.

关 键 词：显齿蛇葡萄 转录组 UNIGENE 功能注释 SSR 

分 类 号：S567.19[农业科学—中草药栽培]