肠道吸收细胞模型研究进展及其在类胡萝卜素上的应用  

Progress in Research and Application of Intestinal Absorption Cell Models on Carotenoids

作　　者：吴帆 陈楠 吴雨卿 倪元颖[1] 温馨[1] WU Fan;CHEN Nan;WU Yuqing;NI Yuanying;WEN Xin(School of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2023年第1期327-336,共10页Food Science

基　　金：国家自然科学基金青年科学基金项目(32101865)。

摘　　要：膳食化合物在维持人体生理功能和预防疾病等方面至关重要,但其必须被人体高效吸收利用才能充分发挥其功能活性。肠道吸收细胞模型已成为体外模拟人体膳食化合物吸收利用及机制研究的有效手段。针对不同的膳食化合物,选取合理的肠道细胞吸收模型以及在现有细胞模型的基础上进一步改进,对于实现高效模拟人体对该膳食化合物的吸收利用具有重要意义。因此,本文概述了人体肠道上皮细胞的结构和功能,系统地综述了体外肠道细胞吸收模型研究进展,并以类胡萝卜素为代表,阐述了其肠道吸收转运机制,重点探讨了Caco-2单细胞模型和Caco-2/HT29-MTX细胞共培养模型在其吸收利用上的应用,以期为更好地利用肠道细胞吸收模型体外模拟人体对不同膳食化合物的吸收利用提供借鉴。Dietary compounds are crucial in maintaining human physiological functions and preventing diseases,but they can give full play to their functional activities only after being effectively absorbed and utilized by the human body.In vitro intestinal cell models have become an effective means to study the absorption and utilization mechanism of dietary compounds.Selecting the appropriate intestinal cell model and improving the existing intestinal cell models are of great significance to effectively simulate the absorption and utilization of dietary compounds in human body.In this paper,the structure and function of human intestinal epithelial cells are introduced,and recent progress in research on in vitro intestinal cell models is systematically reviewed.The intestinal absorption and transport mechanism of carotenoids is expounded,and the focus of this review is on the application of the Caco-2 single cell model and the Caco-2/HT29-MTX co-culture cell model in research on the absorption and utilization of carotenoids,so as to provide a reference for better simulation of absorption and utilization of dietary compounds in human body by using the in vitro intestinal cell absorption models.

关 键 词：体外肠道细胞模型 Caco-2单细胞模型 Caco-2/HT29-MTX细胞共培养模型 类胡萝卜素 吸收利用 

分 类 号：TS201.4[轻工技术与工程—食品科学]