兰州百合多糖的酶解工艺及其体外抗氧化活性研究  

Study on the Enzymatic Hydrolysis of Lanzhou Lily Polysaccharide and its Antioxidant Activity in Vitro

作　　者：惠和平[1] 王萍 张心驰 蔡伟[1] 李晓东[3] 李冠男 刘颖楠 HUI Heping;WANG Ping;ZHANG Xinchi;CAI Wei;LI Xiaodong;LI Guannan;LIU Yingnan(Gansu Vocational College of Agriculture,Lanzhou,Gansu 730020,China;School of Pharmacy,Gansu University of Traditional Chinese Medicine,Lanzhou,Gansu 730010,China;Gansu Hospital of Traditional Chinese Medicine,Lanzhou,Gansu 730050,China)

机构地区：[1]甘肃农业职业技术学院,甘肃兰州730020 [2]甘肃中医药大学药学院,甘肃兰州730010 [3]甘肃省中医院,甘肃省中医药研究院,甘肃兰州730050

出　　处：《农产品加工》2022年第24期23-28,共6页Farm Products Processing

基　　金：甘肃省高等学校创新基金项目(2021A-260);国家自然科学基金项目(82160818)。

摘　　要：以兰州百合多糖为原料,采用β-甘露聚糖酶进行酶解,通过单因素试验与L9(33)正交试验研究底物质量分数、酶添加量、酶解时间对还原糖生成量的影响,优化得出最佳工艺条件;同时以维C为对照,运用DPPH、ABTS自由基、羟自由基和超氧自由基试验来评价兰州百合多糖酶解产物的抗氧化活性。结果表明,兰州百合多糖的最佳酶解工艺为底物质量分数0.2%,酶添加量0.5%,酶解时间4 h,在此条件下还原糖的生成量为0.8062±0.0084 mg/mL。所得酶解产物对4种自由基的清除能力在试验浓度范围内均强于未经酶解兰州百合多糖的清除能力,且呈一定的质量浓度依赖关系。当质量浓度为4 mg/mL时,兰州百合多糖对羟自由基的清除率为69.16%,酶解后产物对羟自由基的清除率达92.18%,接近于阳性对照维C。酶解提高了兰州百合多糖的抗氧化活性。Using Lanzhou lily polysaccharide as raw material,it was hydrolyzed byβ-mannanase.The effects of substrate concentration,enzyme dosage and hydrolysis time on the yield of reducing sugar were studied by single factor test and L9(33)orthogonal test,and the optimal technological condition was obtained.Meanwhile,the DPPH,ABTS free radical,hydroxyl radical and superoxide radical experiments were conducted to evaluate the antioxidant activity of the enzymatic hydrolysis products of Lanzhou lily polysaccharide,with VC as the control.The results showed that the optimal enzymatic hydrolysis condition was substrate concentration 0.2%,enzyme dosage 0.5%,and enzymatic time 4 h.Under these conditions,the yield of reducing sugar was 0.8062±0.0084 mg/mL.The scavenging ability of the obtained enzymatic hydrolysis products to the four free radicals was stronger than that of un-enzymatic hydrolyzing Lanzhou lily polysaccharide in the experimental concentration range,and the scavenging ability was in a certain concentration-dependent manner.When the concentration was 4 mg/mL,the scavenging rate of un-enzymatic hydrolyzing Lanzhou lily polysaccharide to hydroxyl radicals was 69.16%,and the scavenging rate of products after enzymatic hydrolysis to hydroxyl radicals was 92.18%,which was close to the positive control VC.Enzymatic hydrolysis enhanced the antioxidant activity of Lanzhou lily polysaccharide.

关 键 词：兰州百合 多糖 酶解工艺 抗氧化活性 

分 类 号：TQ461[化学工程—制药化工] R284.2[医药卫生—中药学]