芝麻酚对体外培养人结肠癌HCT-8细胞的影响及其机制  

Effects of Sesamol on human colon cancer HCT-8 cells cultured in vitro and its mechanism

作　　者：刘艳波 张宝成 LIU Yanbo;ZHANG Baocheng(3rd Oncology Department of Jiamusi City Cancer Hospital,Jiamusi 154000 Heilongjiang,China;Basic Medical College of Jiamusi University,Jiamusi 154007 Heilongjiang,China)

机构地区：[1]佳木斯市肿瘤医院肿瘤内三科,黑龙江佳木斯154000 [2]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《中国民康医学》2023年第1期1-4,共4页Medical Journal of Chinese People’s Health

基　　金：黑龙江省卫生健康委科研课题(2019—330)。

摘　　要：目的:探讨芝麻酚对体外培养人结肠癌HCT-8细胞的影响及其机制。方法:体外培养人结肠癌HCT-8细胞,分为对照组、芝麻酚50μmol/L组、芝麻酚100μmol/L组和芝麻酚250μmol/L组共4组,采用四甲基偶氮唑盐(MTT)比色法检测各组不同作用时间细胞增殖率,采用流式细胞术检测各组细胞凋亡率,采用蛋白免疫印迹法(Western blotting)检测各组Janus激酶2/信号转导与转录激活因子3(JAK2/STAT3)信号通路相关蛋白、增殖相关蛋白和凋亡相关蛋白表达水平。结果:经芝麻酚处理24、48、72 h后,HCT-8细胞的OD值从高到低依次为对照组>芝麻酚50μmol/L组>芝麻酚100μmol/L组>芝麻酚250μmol/L组,HCT-8细胞的抑制率从低到高依次为对照组<芝麻酚50μmol/L组<芝麻酚100μmol/L组<芝麻酚250μmol/L组,差异有统计学意义(P<0.05);经芝麻酚处理后HCT-8细胞凋亡率从低到高依次为对照组<芝麻酚50μmol/L组<芝麻酚100μmol/L组<芝麻酚250μmol/L组,差异有统计学意义(P<0.05);各组JAK2/GAPDH和STAT3/GAPDH蛋白表达水平比较,差异均无统计学意义(P>0.05);芝麻酚处理后HCT-8细胞中p-JAK2/JAK2和p-STAT3/STAT3蛋白表达水平从高到低依次为对照组>芝麻酚50μmol/L组>芝麻酚100μmol/L组>芝麻酚250μmol/L组,差异有统计学意义(P <0.05);芝麻酚处理后HCT-8细胞中细胞周期蛋白D1(cyclin D1)、c-Myc和B细胞淋巴瘤-2(Bcl-2)蛋白表达水平从高到低依次为对照组>芝麻酚50μmol/L组>芝麻酚100μmol/L组>芝麻酚250μmol/L组,差异有统计学意义(P<0.05);芝麻酚处理后HCT-8细胞中Bax蛋白表达水平从低到高依次为对照组<芝麻酚50μmol/L组<芝麻酚100μmol/L组<芝麻酚250μmol/L组,差异有统计学意义(P<0.05)。结论:不同剂量芝麻酚均对体外培养人结肠癌HCT-8细胞具有抑制增殖、诱导凋亡作用,其机制可能与抑制JAK2/STAT3信号通路的激活有关。Objective: To investigate effects of Sesamol on human colon cancer HCT-8 cells cultured in vitro and its mechanism. Methods: Human colon cancer HCT-8 cells were cultured in vitro and were divided into four groups: control group, 50 μmol/L Sesamol group, 100 μmol/L Sesamol group and 250 μmol/L Sesamol group. The cell proliferation rate of each group was detected and compared by methyl thiazolyl tetrazolium(MTT)colorimetric method, and the apoptosis rate of each group was detected and compared by flow cytometry. Western blotting was used to detect and compare the expression levels of JAK2/STAT3 signaling pathway-related proteins, proliferation-related proteins and apoptosis-related proteins in each group at different time points. Results: After treatment with Sesamol for 24,48 and 72 h, the OD value of HCT-8 cells from high to low was control group > 50 μmol/L Sesamol group > 100 μmol/L Sesamol group > 250 μmol/L Sesamol group;the inhibition rate of HCT-8 cells from low to high was control group < 50 μmol/L Sesamol group < 100 μmol/L Sesamol group < 250 μmol/L Sesamol group;and the differences were statistically significant(P<0.05). The apoptosis rate of HCT-8 cells treated with Sesamol from low to high was control group < 50 μmol/L Sesamol group < 100 μmol/L Sesamol group < 250 μmol/L Sesamol group, and the differences were statistically significant(P<0.05). There were no significant differences in the expression levels of JAK2/GAPDH and STAT3/GAPDH in each group(P>0.05). The expression levels of p-JAK2/JAK2 and p-STAT3/STAT3 in HCT-8 cells treated with Sesamol from high to low were control group > 50 μmol/L Sesamol group > 100 μmol/L Sesamol group > 250 μmol/L Sesamol group, and the differences were statistically significant(P<0.05). The expression levels of cyclin D1, c-Myc and Bcl-2 in HCT-8 cells treated with Sesamol were control group > 50 μmol/L Sesamol group > 100 μmol/L Sesamol group > 250 μmol/L Sesamol group, and the differences were statistically significant(P<0.05). Further, the expre

关 键 词：芝麻酚 结肠癌 体外培养 人HCT-8细胞 JAK2/STAT3信号通路 细胞周期蛋白D1 

分 类 号：R735[医药卫生—肿瘤]