刺梨与无籽刺梨果实中药化学成分及抗氧化物质的比较分析  

Comparative analysis of chemical components of traditional Chinese medicine and antioxidants between Rosa roxburghii and R.sterilis fruits

作　　者：蒋兰兰 鲁敏[1] 安华明[1] JIANG Lanlan;LU Min;AN Huaming(Agricultural College,Guizhou University/Guizhou Engineering Research Center for Fruit Crops,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州大学农学院·贵州省果树工程技术研究中心,贵阳550025

出　　处：《果树学报》2023年第1期111-125,共15页Journal of Fruit Science

基　　金：国家自然科学基金委员会-贵州省人民政府喀斯特科学研究中心项目(U1812401);国家自然科学基金(32060657);贵州省科技计划项目(黔科合支撑[2021]一般242)。

摘　　要：【目的】明确刺梨(Rosa roxburghii Tratt.)与无籽刺梨(R.sterilis S.D.Shi)果实的次生代谢物中的中药化学成分及抗氧化物质并分析其间差异,促进刺梨与无籽刺梨的合理应用。【方法】采用超高效液相色谱串联质谱技术(UPLCMS/MS)对刺梨2个基因型(Rr-3,Rr-5)及无籽刺梨(Rs)果实的次生代谢物进行定性定量分析,筛选鉴定并比较分析两类果实中的中药化学成分及抗氧化代谢物差异。【结果】2类果实中共检测到12类530种次生代谢物,31种仅在刺梨中检测到,4种仅在无籽刺梨中检测到;其中118种代谢物属于TCMSP登记的中药化学成分,而145种物质与抗氧化能力显著相关。差异代谢物比较分析发现,Rr-3与Rs间有272种差异代谢物,Rr-5与Rs间有242种差异代谢物;两个刺梨基因型与Rs有171种共有差异代谢物,其中111种物质在刺梨果实中含量更高,包括31种中药化学成分和8种抗氧化物质;无籽刺梨中有53种物质含量更高,其中包含10种中药化学成分。进一步的果实抗氧化能力分析发现,刺梨果实的DPPH、ABTS及FRAP等3种抗氧化能力均显著高于无籽刺梨。【结论】刺梨果实较无籽刺梨具更强的中药活性及抗氧化能力,从而具有更高的医药保健价值及功能食品开发潜力。【Objective】Rosa roxburghii Tratt.(Rr)and R.sterilis S.D.Shi(Rs)are both deciduous fruit crops of Rosaceae,and they are rich in a variety of bioactive compounds,including L-ascorbic acid,phenlic acid,organic acid,amino acid,triterpenes and flavonoids,so that they have high nutritional and medicinal value as well as antioxidant capacity.There are significant differences in nutritional components,pharmacological activities and antioxidant capacity between Rr and Rs fruits.However,the chemical components of traditional Chinese medicine and antioxidants in two kinds of fruits and the differences between them are not clear.Therefore,this study will identify and analyze the secondary metabolites,chemical components of traditional Chinese medicine,antioxidants and their differences between Rr and Rs fruits,so as to promote the rational application of Rr and Rs.【Methods】Ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)was used to qualitatively and quantitatively analyze the secondary metabolites of two R.roxburghii genotypes(Rr-3;Rr-5)and R.sterilis(Rs)fruits.Searching and identifying the chemical components of traditional Chinese medicine in secondary metabolites were carried out on TCMSP platform(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform).The ultraviolet spectrophotometry was employed to detect the capacity of three antioxidants(DPPH,1,1-diphenyl-2-picrylhydrazyl;FRAP,fluorescence recovery after photobleaching;ABTS,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid).Identification of antioxidant substances was confirmed by correlation analysis between secondary metabolites and three antioxidant capacities(DPPH,FRAP,ABTS).The differences in the chemical components of traditional Chinese medicine and antioxidant metabolites between two types of fruits were screened,identified and compared.【Results】A total of 530 secondary metabolites belonging to 12 categories were detected(99 lipids,90 phenolic acids,64 amino acids and derivatives,62

关 键 词：刺梨 无籽刺梨 次生代谢物 中药化学成分 抗氧化物质 

分 类 号：S661.9[农业科学—果树学]