红毛藻不同乙醇浓度提取物的生物活性及其成分分析  

Bioactivity and Component Analysis of Different Ethanol Concentration Extracts from Bangia Fusco-purpurea

作　　者：常高萍 林巧燕 张敏[1] 郭佳瑄 李志朋 杜希萍[1,2,3,4] 姜泽东 CHANG Gao-ping;LIN Qiao-yan;ZHANG Min;GUO Jia-huan;LI Zhi-peng;DU Xi-ping;JIANG Ze-dong(College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China;Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering,Xiamen 361021,China;Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China;Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed of Southern Ocean Technology Center of Xiamen,Xiamen 361021,China)

机构地区：[1]集美大学海洋食品与生物工程学院,福建厦门361021 [2]福建省食品微生物与酶工程重点实验室,福建厦门361021 [3]厦门市食品与生物工程技术研究中心,福建厦门361021 [4]厦门南方海洋研究中心海藻资源化利用与深加工重点实验室,福建厦门361021

出　　处：《化学试剂》2023年第2期98-105,共8页Chemical Reagents

基　　金：国家自然科学基金项目(22038012);福建省自然科学基金项目(2021J01833)。

摘　　要：为了探究红毛藻乙醇提取物活性成分及其功能活性,使用不同浓度乙醇溶液提取红毛藻,并测定不同浓度乙醇溶液提取物中的总多酚、总生物碱、总黄酮含量。通过研究胰脂肪酶、酪氨酸酶、乙酰胆碱酯酶抑制活性和DPPH自由基清除能力来评价不同浓度乙醇溶液提取红毛藻的活性差异。通过LC-MS/MS技术完成红毛藻乙醇提取物中的多酚鉴定。结果表明,红毛藻不同乙醇浓度提取物均具有DPPH清除能力以及酪氨酸酶、胰脂肪酶、乙酰胆碱酯酶抑制活性,乙醇浓度不同,其提取物生物活性不同,总多酚、总黄酮、总生物碱含量不同;其中100%乙醇浓度提取物总多酚、总黄酮和总生物碱含量最高,具有较强的DPPH清除能力和酪氨酸酶抑制活性。同时结果还表明,总多酚和总黄酮含量与酪氨酸酶抑制活性和DPPH清除能力均具有极显著相关性。LC-MS/MS结果显示红毛藻100%乙醇提取物中含有37种多酚类化合物。For exploring the active constituents and functions of ethanol extracts from Bangia fusco-purpurea,the contents of total polyphenols,alkaloids and flavonoids were determined.The inhibitory effects of pancreatic lipase,tyrosinase and acetylcholinesterase and DPPH free radical scavenging were evaluated.The identification of polyphenols in ethanolic extracts of B.fusco-purpurea was completed by LC-MS/MS.The results showed that different ethanol concentration extracts from B.fusco-purpurea had different DPPH scavenging ability,tyrosinase,pancreatic lipase and acetylcholinesterase inhibitory activity,and different contents of total polyphenols,flavonoids,alkaloids.100%ethanol extract from B.fusco-purpurea had the highest contents of total polyphenols,flavonoids,alkaloids,and stronger DPPH scavenging ability and tyrosinase inhibitory activity.Correlation analysis showed that the contents of total polyphenols and flavonoids were significantly correlated with tyrosinase inhibitory activity and DPPH scavenging ability.LC-MS/MS results revealed 37 polyphenolic compounds in 100%ethanolic extract from B.fusco-purpurea.

关 键 词：红毛藻 生物活性 成分分析 相关性分析 LC-MS/MS 

分 类 号：TS201.1[轻工技术与工程—食品科学]