miR-181b promotes angiogenesis and neurological function recovery after ischemic stroke  

作　　者：Li-Xia Xue Lin-Yuan Shu Hong-Mei Wang Kai-Li Lu Li-Gang Huang Jing-Yan Xiang Zhi Geng Yu-Wu Zhao Hao Chen 

机构地区：[1]Department of Neurology,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China [2]Department of Emergency Medicine,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China [3]Department of Neurosurgery,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China

出　　处：《Neural Regeneration Research》2023年第9期1983-1989,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,Nos.81801169 (to LXX),82071404 (to HC),81870952 (to HMW)。

摘　　要：Promotion of new blood vessel formation is a new strategy for treating ischemic stroke.Non-coding miRNAs have been recently considered potential therapeutic targets for ischemic stroke.miR-181b has been shown to promote angiogenesis in hypoxia and traumatic brain injury model,while its effect on ischemic stroke remains elusive.In this study,we found that overexpression of miR-181b in brain microvascular endothelial cells subjected to oxygen-glucose deprivation in vitro restored cell prolife ration and enhanced angiogenesis.In rat models of focal cerebral ischemia,ove rexpression of miR-181b reduced infarction volume,promoted angiogenesis in ischemic penumbra,and improved neurological function.We further investigated the molecular mechanism by which miR-181b participates in angiogenesis after ischemic stroke and found that miR-181b directly bound to the 3’-UTR of phosphatase and tensin homolog(PTEN) mRNA to induce PTEN downregulation,leading to activation of the protein kinase B(Akt) pathway,upregulated expression of vascular endothelial growth facto rs,down-regulated expression of endostatin,and promoted angiogenesis.Taken togethe r,these results indicate that exogenous miR-181b exhibits neuroprotective effects on ischemic stro ke through activating the PTEN/Akt signal pathway and promoting angiogenesis.

关 键 词：Akt ANGIOGENESIS ENDOSTATIN ischemic stroke middle cerebral artery occlusion miR-181b neurological function recovery oxygen-glucose deprivation PTEN vascular endothelial growth factor 

分 类 号：R743.31[医药卫生—神经病学与精神病学]