MUTYH is a potential prognostic biomarker and correlates with immune infiltrates in hepatocellular carcinoma  

作　　者：Fan Yang Qinghai Lian Beibei Ni Xiusheng Qiu Yizhan He Xiaoguang Zou Fangping He Wenjie Chen 

机构地区：[1]Biotherapy Center,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou,Guangdong,China [2]Department of Infectious Diseases,The First People's Hospital of Kashi,Kashi,Xinjiang,China [3]Xinjiang Medical University,Urumqi,Xinjiang,China [4]Cell-gene Therapy Translational Medicine Research Center,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou,Guangdong,China [5]Department of Hepatobiliary and Pancreatic Surgery,The Eighth Affiliated Hospital of Sun Yat-sen University,Shenzhen,Guangdong,China

出　　处：《Liver Research》2022年第4期258-268,共11页肝脏研究（英文）

基　　金：This work was supported by a project funded by the National Natural Science Foundation of China (82260110,81870449,82170674,51933011);China Postdoctoral Science Foundation (2019M653904XB);Natural Science Foundation of Xinjiang Uyghur Autonomous Region (2020D01C006).

摘　　要：Background:Hepatocellular carcinoma(HCC)is a leading cause of cancer-related death worldwide.The development of biomarkers for early detection and monitoring of HCC has not shown significant prog-ress.Meanwhile,the second adenomatous polyposis-related gene,MUTYH,which encodes a DNA gly-cosylase,has been observed in its contribution to oxidative DNA damage repair.Abnormal expression of MUTYH can reduce cell survival rate.Therefore,this study investigated the usefulness of MUTYH in diagnosing and prognosis HCC.Materials and methods:Using The Cancer Genome Atlas(TCGA)data,we analyzed the prognostic value of MUTYH in HCC.We used logistic regression,Wilcoxon signed-rank test,and KruskaleWallis test to examine MUTYH expression concerning clinical-pathologic characteristics.Univariate and multivariate Cox regression methods and Kaplan-Meier analysis were applied to determine the related prognostic factors of HCC.The enrichment analysis(GSEA)was used to determine the critical pathways associated with MUTYH.The single-sample gene set enrichment analysis(ssGSEA)was conducted to examine the correlation between MUTYH expression and cancer immune infiltration.Results:The higher expression of MUTYH in HCC patients was associated with a poorer overall survival rate and a shorter disease-specific survival rate.The Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis showed that all differentially expressed genes(DEGs)between the high and low expression levels of MUTYH significantly enriched in the trace ligand-receptor interaction,cell cycle,oocyte meiosis,gap junction,and DNA replication.Group analysis revealed the signals of their open access.The neuron system,M phase,DNA repair,Rho GTPase effector,and cell cycle checkpoints were significantly enriched.ssGSEA showed a positive correlation between MUTYH expression and the infiltration levels of Th2 cells,NK cells,and T helper cells.Moreover,a negative correlation was found between MUTYH expression and the infiltration levels of dendritic cells(DCs)and cytotoxic cells.Conclusio

关 键 词：MUTYH Hepatocellular carcinoma(HCC) BIOINFORMATICS Prognostic biomarker Immune infiltrates 

分 类 号：R735.7[医药卫生—肿瘤]