Antioxidant procyanidin B2 protects oocytes against cryoinjuries via mitochondria regulated cortical tension  

作　　者：Qingrui Zhuan Jun Li Xingzhu Du Luyao Zhang Lin Meng Yuwen Luo Dan Zhou Hongyu Liu Pengcheng Wan Yunpeng Hou Xiangwei Fu 

机构地区：[1]Key Laboratory of Animal Genetics,Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs,Key Laboratory for Animal Genetic Improvement,College of Animal Science and Technology,National Engineering Laboratory for Animal Breeding,China Agricultural University,Beijing,China. [2]Department of Reproductive Medicine,Reproductive Medical Center,The First Hospital of Hebei Medical University,Shijiazhuang,Hebei,China. [3]State Key Laboratories of Agrobiotechnology,College of Biological Sciences,China Agricultural University,Beijing,China. [4]State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding,Institute of Animal Husbandry and Veterinary Sciences,Xinjiang Academy of Agricultural and Reclamation Sciences,Shihhotze,China.

出　　处：《Journal of Animal Science and Biotechnology》2023年第1期166-187,共22页畜牧与生物技术杂志（英文版）

基　　金：National Key Research and Development Program Topics,Grant/Award Number:2021YFD1200402;Chinese Universities Scientific Fund,Grant/Award Number:2021TC061;Natural Science Foundation of Hebei province,Grant/Award Number:H2020206254;Special Program for Training and Guiding Outstanding Young and Middle-aged Talents,Grant/Award Number:SKLSGIHP2021A01;National Natural Science Foundation of China,Grant/Award Number:81901562&31372307;Key research and development projects in Hebei province,Grant/Award Number:18226604D;Program of Young and Middle-aged Scientific and technological Innovation Leaders of the Xinjiang Production and Construction Corps,Grant/Award Number:2018CB025;Xinghuo program of the First Hospital of Hebei Medical University,Grant/Award Number:XH202005;The Central Guidance on Local Science and Technology Development Fund of Hebei Province,Grant/Award Number:226Z7713G。

摘　　要：Background:Irreversible cryodamage caused by oocyte vitrification limited its wild application in female fertility preservation.Antioxidants were always used to antagonist the oxidative stress caused by vitrification.However,the comprehensive mechanism underlying the protective role of antioxidants has not been studied.Procyanidin B2(PCB2)is a potent natural antioxidant and its functions in response to vitrification are still unknown.In this study,the effects of PCB2 on vitrified-thawed oocytes and subsequent embryo development were explored,and the mechanisms underlying the protective role of PCB2 were systematically elucidated.Results:Vitrification induced a marked decline in oocyte quality,while PCB2 could improve oocyte viability and further development after parthenogenetic activation.A subsequent study indicated that PCB2 effectively attenuated vitrification-induced oxidative stress,rescued mitochondrial dysfunction,and improved cell viability.Moreover,PCB2 also acts as a cortical tension regulator apart from strong antioxidant properties.Increased cortical tension caused by PCB2 would maintain normal spindle morphology and promote migration,ensure correct meiosis progression and finally reduce the aneuploidy rate in vitrified oocytes.Further study reveals that ATP biosynthesis plays a crucial role in cortical tension regulation,and PCB2 effectively increased the cortical tension through the electron transfer chain pathway.Additionally,PCB2 would elevate the cortical tension in embryo cells at morula and blastocyst stages and further improve blastocyst quality.What's more,targeted metabolomics shows that PCB2 has a beneficial effect on blastocyst formation by mediating saccharides and amino acids metabolism.Conclusions:Antioxidant PCB2 exhibits multi-protective roles in response to vitrification stimuli through mitochondria-mediated cortical tension regulation.

关 键 词：Cortical tension MEIOSIS MITOCHONDRIA OOCYTES Procyanidin B2 VITRIFICATION 

分 类 号：S8[农业科学—畜牧兽医]