尿素包合法从葡萄籽油中富集高纯度亚油酸甲酯及包合物表征分析  

Enrichment of high purity methyl linoleate from grape seed oil by urea adduction and characterization of adduction complexes

作　　者：曾俊 岳杨 任小娜 茹仙古丽·尤努斯 姜蕾 ZENG Jun;YUE Yang;REN Xiaona;RUXIANGULI Younusi;JIANG Lei(College of Life and Geographic Sciences,Kashi University,Kashi 844000,Xinjiang,China;Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region,Kashi 844000,Xinjiang,China)

机构地区：[1]喀什大学生命与地理科学学院,新疆喀什844000 [2]新疆帕米尔高原生物资源与生态重点实验室,新疆喀什844000

出　　处：《中国油脂》2022年第12期25-30,共6页China Oils and Fats

基　　金：新疆维吾尔自治区科技计划项目(2021D01B07);喀什大学校内课题(19-2645)。

摘　　要：为提升葡萄籽的使用价值,以葡萄籽油为原料,采用尿素包合法富集高纯度亚油酸甲酯。研究了脂肪酸甲酯与尿素质量比、尿素与95%乙醇质量比、包合时间和包合温度对包合效果的影响,并采用傅里叶变换红外光谱(FTIR)、X射线衍射(XRD)和扫描电子显微镜(SEM)对空白尿素结晶物和包合固形物进行表征。结果表明:制备高纯度亚油酸甲酯的最佳工艺条件为脂肪酸甲酯与尿素质量比1∶2、尿素与95%乙醇质量比1∶4、包合温度5℃、包合时间10 h,在此条件下得到的亚油酸甲酯含量为94.83%,收率为40.41%;FTIR和XRD分析表明,尿素包合反应没有新的官能团生成,且脂肪酸甲酯的包合降低了尿素的结晶度;SEM图像显示,尿素与脂肪酸甲酯形成了六棱体包合物。综上,尿素包合法为富集葡萄籽油中的高纯度亚油酸甲酯的有效方法。To improve the value of grape seed,high purity methyl linoleate was prepared from grape seed oil by urea adduction.The effects of the mass ratio of fatty acid methyl ester to urea,mass ratio of urea to 95%ethanol,adduction time and adduction temperature were studied.The control urea crystal and adduction solid were characterized by Fourier transform infrared spectroscopy(FTIR),X-ray diffraction(XRD)and scanning electron microscope(SEM).The optimum conditions for the preparation of high purity methyl linoleate were obtained as follows:mass ratio of fatty acid methyl ester to urea 1∶2,mass ratio of urea to 95%ethanol 1∶4,adduction temperature 5℃,adduction time 10 h.Under the optimum conditions,the content of methyl linoleate was 94.83%,and the yield was 40.41%.FTIR and XRD analysis showed that no new functional group was formed in urea adduction,and the crystallinity of urea was reduced by the adduction of methyl linoleate.SEM images confirmed the formation of hexahedral adduction complex between urea and fatty acid methyl ester.In summary,urea adduction is an effective method for enrichment of high purity methyl linoleate from grape seed oil.

关 键 词：葡萄籽油 尿素包合 亚油酸甲酯 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ645.6[轻工技术与工程—食品科学与工程]