STUDYING THE ROLE OF MACROPHAGES IN CIRCULATING PROSTATE CANCER CELLS BY IN VIVO FLOW CYTOMETRY  

作　　者：JIN GUO ZHICHAO FAN ZHENGQIN GU XUNBIN WEI 

机构地区：[1]Department of Chemistry,Fudan University,Shanghai,P.R.China [2]Institutes of Biomedical Sciences,Fudan University,Shanghai,P.R.China [3]Med-X Research Institute,Shanghai Jiao Tong University 1954,Huashan Road,Shanghai 200240,P.R.China [4]School of Biomedical Engineering Shanghai Jiao Tong University,Shanghai,P.R.China [5]Department of Urology,Xinhua Hospital Shanghai Jiao Tong University 1665,Kongjiang Road,Shanghai 200092,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2012年第4期39-47,共9页创新光学健康科学杂志（英文）

基　　金：supported by the National Basic Research Program of China(973Program,2011CB910404 and 2012CB966800);China National Natural Science Foundation(30901432 and 30972949);the Chinese Ministry of Education(109056);Program for New Century Excellent Talents in University Award(NCET-08-0131);Shanghai Science and Tech-nology Committee(11DZ2211000).

摘　　要：Metastasis is a very complicated multi-step process and accounts for the low survival rate of the cancerous patients.To metastasize,t he malignant cells must detach from the primary tumor and migrate to secondary sites in the body through either blood or lymph circulation.Macrophages appear to be directly involved in tumor progression and metastasis.However,the role of macrophages in affecting cancer metast asis has not been fully elucidated.Here,we have utilized an emerging technique,namely in vivo flow cytometry(IVFC)to study the depletion kinetics of circulating prostate cancer cells in mice and determine how depletion of macrophages by the liposome encapsulated clodronate affects the depletion kinetics.Our results show diferent depletion kinetics of PC-3 cells between the macrophagedeficient group and the control group.The number of circulating tumor cells(CTCs)in the macrophage-deficient group decreases in a slower manner compared to the control mice group.The differences in depletion kinetics indicate that the absence of macrophages facilitates the stay of prostate cancer cells in circulation.In addition,our imaging data suggest that macrophages might be able to arrest,phagocytose and digest PC-3 cells.Therefore,phagocy tosis may mainly contribute to the de-pletion kinetic diferences.The developed methods elaborated here would be useful to study the relationship between macr ophages and tumor metastasis in small animal cancer models.

关 键 词：Prostate cancer MACROPHAGES liposome-encapsulated clodronate in vrivo flow cytometer circulating tumor cells 

分 类 号：R73[医药卫生—肿瘤]