Studies on photodynamic mechanism of a novel chlorine derivative(TDPC)and its antitumor effect forphotodynamic therapy in vitro and in vivo  

作　　者：Ying Ye Lai-Xing Wang Dan-Ping Zhang Yi-Jia Yan Zhi-Long Chen 

机构地区：[1]Department of Pharmaceutical Science&Technology College of Chemistry and Biology,Donghua University Shanghai 201620,P.R.China [2]Changhai Hospital,Second Military Medical University Shanghai 200433,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2015年第1期60-67,共8页创新光学健康科学杂志（英文）

基　　金：supported by Chinese National Natural Science Foundation (21372042,81301878,81101298);Foundation of Shanghai government (13431900700,13430722300,13ZR1441000,13ZR1440900);Foundation of Donghua University (No.11D10501,12D10515);Foundation of Yiwu Science and Technology Bureau (2011-G1-15,2012-G3-02).

摘　　要：Photodynamic therapy(PDT)represents a promising method for treatment of cancerous tumors.The chemical and physical properties of used photosensitizer(PS)play key roles in the treat ment efficacy.In this study,a novel PS,5,10,15,20-tetrais((5-dipropylamino)pentyl)-chlorin(TDPC)which displayed a characteristic long wavelength absorption peak at 650 nm were synthesized.It also shows a singlet oxygen generation rate of 4.257 min^(-1).Generally,TDPC is localized in mitochondria and nucleus of cell.After light irradiation with 650 nm laser,it can kill many types of cell,in addition,TDPC-PDT can destroy ECA-109 tunor in nude mice and a necrotic scab was formed eventually.The expression levels of many genes which regulated cell growth and apoptosis were determined by RT-PCR following TDPC-PDT.The results showed that it either increased or decreased,among which,the expression level of TNFSF13,a member of tumor necrosis factor superfamily,increased signifcantly.In general,TDPC is an elective antitumor PS in vitro and in vito and is worthy of further study as a new drug candidate.TNFSF13 will be an important molecular target for the discovery of new PSs.

关 键 词：PHOTOSENSITIZER photodynamic therapy TUMOR CHLORIN TDPC 

分 类 号：R73[医药卫生—肿瘤]