IMAGING REDOX STATE HETEROGENEITY WITHIN INDIVIDUAL EMBRYONIC STEM CELL COLONIES  

作　　者：HE N.XU RUSSELL C.ADDIS DAVIDA F.GOINGS SHOKO NIOKA BRITTON CHANCE JOHN D.GEARHART LIN Z.LI 

机构地区：[1]Molecular Imaging Laboratory,Department of Radiology University of Pennsylvania,School of Medicine Philadelphia,PA 19104,USA [2]Institute for Regenerative Medicine and Department of Cell and Developmental Biology University of Pennsylvania,School of Medicine Philadelphia,PA 19104,USA [3]Britton Chance Laboratory of Redox Imaging Johnson Research Foundation Department of Biochemistry and Biophysics University of Pennsylvania,School of Medicine Philadelphia,PA 19104,USA [4]Institute of Translational Medicine and Therapeutics University of Pennsylvania,Philadelphia,PA 19104,USA

出　　处：《Journal of Innovative Optical Health Sciences》2011年第3期279-288,共10页创新光学健康科学杂志（英文）

基　　金：provided by the Institute for Regenerative Medicine at University of Pennsylvania with an IRM pilot grant(L.Z.Li).

摘　　要：Redox state mediates embryonic stem cell(ESC)differentiation and thus offers an important complementary approach to understanding the pluripotency of stem cells.NADH redox ratio(NADH/(Fp t NADH)),where NADH is the reduced form of nicotinamide adenine dinucleotide and Fp is the oxidizedflavoproteins,has been established as a sensitive indicator of mitochondrial redox state.In this paper,we report our redox imaging data on the mitochondrial redox state of mouse ESC(mESC)colonies and the implications thereof.The low-temperature NADH/Fp redox scanner was employed to image mESC colonies grown on a feeder layer of gamma-irradiated mouse embryonicfibroblasts(MEFs)on glass cover slips.The result showed significant heterogeneity in the mitochondrial redox state within individual mESC colonies(size:~200-440μm),exhibiting a core with a more reduced state than the periphery.This more reduced state positively correlates with the expression pattern of Oct4,a well-established marker of pluripotency.Our observation is thefirst to show the heterogeneity in the mitochondrial redox state within a mESC colony,suggesting that mitochondrial redox state should be further investigated as a potential new biomarker for the stemness of embryonic stem cells.

关 键 词：Redox imaging PLURIPOTENCY NADH FLAVOPROTEINS redox ratio 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]