KillerRed protein based in vivo photodynamic therapy and corresponding tumor metabolic imaging  

作　　者：Qiaoya Lin Shuang Sha Fei Yang Honglin Jin Zhihong Zhang 

机构地区：[1]Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074,P.R.China [2]MoE Key Laboratory for Biomedical Photonics Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2016年第1期21-30,共10页创新光学健康科学杂志（英文）

基　　金：This work was supported by the Major Research plan of the National Natural Science Foundation of China (Grant No.91442201);the China Postdoctoral Science Foundation funded project (Grant No.2015M572148,2012M521430 and 2013T60721);the Open Research Fund of State Key Laboratory of Bioelectronics,Southeast University.

摘　　要：Photodynamic therapy(PDT)gains wide attention as a useful therapeutic method for cancer.It is mediated by the oxygen and photosensitizer under the specific light irradiation to produce the reactive oxygen species(ROS),which induce cellular toxicity and regulate the redox potential in tumor cells.Nowadays,genetic photosensitizers of low toxicity and easy production are required to be developed.KillerRed,a unique red fluorescent protein exhibiting excellent phototoxic properties,has the potential to act as a photosensitizer in the application of tumor PDT.Meantime,the course of tumor redox metabolism during this treatment was rarely investigated so far.Thus here,we investigated the effects of KillerRed-based PDT on tumor growth in vivo and examined the subsequent tumor metabolic states including the changes of nicotinamide adenine dinucleotide hydrogen(NADH)and flavoprotein(Fp),two important metabolic coenzymes of tumor cells.Results showed the tumor growth had been significantly inhibited by KillerRedbased PDT treatment compared to control groups.A home-made cryo-imaging redox scanner was used to measure intrinsic fluorescence and exogenous KillerRed fluorescence signals in tumors.The Fp signal was elevated by nearly 4.5-fold,while the NADH signal decreased by 66%after light irradiation,indicating that Fp and NADH were oxidized in the course of KillerRedbased PDT.Furthermore,we also observed correlation between the fluorescence distribution of KillerRed and NADH.It suggests that the KillerRed protein based PDT might provide a new approach for tumor therapy accompanied by altering tumor metabolism.

关 键 词：Reactive oxygen species redox METABOLISM FLAVOPROTEIN nicotinamide adenine dinucleotide hydrogen 

分 类 号：R73[医药卫生—肿瘤]