响应面法优化纤维素酶提取海洋微藻多糖的研究  

Optimization of cellulase extraction of marine microalgae polysaccharide by response Surface methodology

作　　者：林甜甜 徐晓阳 张子聪 陈欣苗 王昱晨 陈文伟 LIN Tiantian;XU Xiaoyang;ZHANG Zicong;CHEN Xinmiao;WANG Yuchen;CHEN Wenwei(School of life sciences,China Jiliang university,Zhejiang Hangzhou 310018,China)

机构地区：[1]中国计量大学生命科学学院,浙江杭州310018

出　　处：《食品工程》2022年第4期66-70,共5页Food Engineering

基　　金：国家级大学生创新创业训练计划资助项目(201810356038)。

摘　　要：海洋微藻中的多糖结构多样,具备多重天然生物活性,是可高度利用的生物资源。以海洋微藻为原料,纤维素酶为酶制剂,通过响应面法优化藻多糖提取的工艺。试验结果表明,纤维素酶提取分离海洋微藻多糖的最佳工艺为:酶添加量2.20%,料液比(g/mL) 1∶42,酶解时间2.2 h,酶解温度49℃,该条件下藻多糖的实际得率达6.17%。这为海洋微藻多糖的深入研究和规模化生产奠定了一定的理论依据和技术基础。The polysaccharides in marine microalgae have various structures and multiple natural biological activities, which can be highly utilized as biological resources. The response surface methodology was used to optimize the extraction process of algae polysaccharide using Marine microalgae as raw material and cellulase as enzyme preparation. The results showed that the optimal extraction conditions were as follows: enzyme dosage 2.20%, solidliquid ratio(g/mL) 1: 42, reaction time 2.2h, reaction temperature 49℃, and the actual extraction rate of polysaccharide from marine micro-algae reached 6.17%. This provided a theoretical and technical basis for the further study and large-scale production of marine micro-algae polysaccharides.

关 键 词：海洋微藻 藻多糖 纤维素酶 响应面优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]