Biomass-based Carbon Dots as Peroxidase Mimics for Colorimetric Detection of Glutathione and L-Cysteine  

作　　者：LIU Junxue WANG Yufei MA Wenyan ZONG Siyu LI Jiyang 

机构地区：[1]State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,College of Chemistry,Jilin University,Changchun,130012,P.R.China

出　　处：《Chemical Research in Chinese Universities》2022年第6期1446-1452,共7页高等学校化学研究（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.21671075, 21621001);the “111” Project of China (No. B17020).

摘　　要：Biomass-carbon dots(named as B-CDs) have been successfully prepared via facile one-step hydrothermal reflux treatment at a low temperature from passion fruit peel. The as-prepared B-CDs had a uniform sphere morphology and size of 1–3 nm, which possessed rich nitrogen/oxygen-containing functional groups. B-CDs could imitate peroxidase and accelerate the reaction process of H2O_(2) oxidizing the substrate 3,3’,5,5’-tetramethylbenzidine(TMB), which caused an obvious color change in the solution due to the oxidization of achromic TMB into blue oxidation product(ox-TMB) with an absorption peak at 652 nm. Furthermore, the product could be further reduced to native colorless TMB by the reducing agent glutathione or L-Cysteine. Thus, depending on the peroxidase-like properties of B-CDs, we developed a colorimetric approach to detecting both glutathione and L-Cysteine, which showed superior selective and sensitive detection towards glutathione and L-Cysteine in a linear range of 0–20 µmol/L. The limits of detection were 0.62 and 0.58 µmol/L, respectively.

关 键 词：Carbon dot Biomass COLORIMETRY GLUTATHIONE CYSTEINE 

分 类 号：O62[理学—有机化学]