扁刺蛾核型多角体病毒新分离株的基因组测序与分析  

Genome Sequencing and Analyses of A New Strain of Thosea sinensis Nucleopolyhedrovirus(OxocNPV-Ts)

作　　者：王金昌[1] 类承凤 陈俊晖 占智高[1] 况文东 关丽梅[1] 杨健 李江怀 周溪 靳亮[1] 王君[2] WANG Jinchang;LEI Chengfeng;CHEN Junhui;ZHAN Zhigao;KUANG Wendong;GUAN Limei;YANG Jian;LI Jianghuai;ZHOU Xi;JIN Liang;WANG Jun(Institute of Microbiology Jiangxi Academy of Sciences,Nanchang 330096,China;State Key Laboratory of Virology and National Virus Resource Center,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China)

机构地区：[1]江西省科学院、微生物研究所,南昌330096 [2]中国科学院、武汉病毒研究所,武汉430071

出　　处：《病毒学报》2023年第1期185-198,共14页Chinese Journal of Virology

基　　金：国家自然科学基金项目(项目号:31900154),题目:深度挖掘杆状病毒蛋白质网络;江西省重点研发项目(项目号:20192BBF60056),题目:草原毛虫多角体病毒复配增效杀虫剂关键技术及应用研究;江西省重点研发项目(项目号:20202BBFL63050),题目:扁刺蛾核型多角体病毒杀虫剂生产关键技术;江西省重点研发项目(项目号:20212BBF63044),题目:豆天蛾质型多角体病毒杀虫剂关键技术研究;江西省科学院包干制试点示范项目(项目号:2021YSBG21013),题目:多功能解淀粉芽孢杆菌发酵优化及其抑菌杀蚜水剂的开发应用。

摘　　要：从自然死亡的扁刺蛾幼虫分离到一株核型多角体病毒。扁刺蛾核型多角体病毒新分离株(Oxyplax ochracea nucleopolyhedrovirus-Thosea sinensis,OxocNPV-Ts)具有典型的杆状病毒特征,呈单粒包埋型病毒粒子,病毒颗粒(Occlusion-derived virus,ODV)呈不规则的多边形,直径为0.63μm~1.49μm。OxocNPV-Ts能有效杀灭扁刺蛾幼虫,它对扁刺蛾幼虫的半致死浓度LC50为4×10^(6)PIB/mL,是一种理想的生物防治剂。本实验对OxocNPV-Ts基因组进行了测序和鉴定。OxocNPV-Ts基因组全长114781 bp,包含124个开放阅读框。它与斜纹刺蛾核型多角体病毒(OxocNPV)关系最为密切,序列相似性高达99%。系统进化分析表明,OxocNPV-Ts属于杆状病毒科alpha杆状病毒属(Alphabaculovirus),根据国际病毒分类委员会(International Committee on Taxonomy of Viruses,ICTV)的种属划分标准,OxocNPV-Ts是OxocNPV的一个株系。新报道的基因组为研究该病毒毒力的分子机制提供了理论基础。We isolated a nucleopolyhedrovirus stain from the naturally diseased larvae of Thosea sinensis Walker.OxocNPV-Ts is a new strain of Thosea sinensis nucleopolyhedrovirus.OxocNPV-Ts shows typical baculovirus characteristics,and is a single embedded virus particle.ODV particles are irregular polygons of diameter 0.63μM-1.49μM.OxocNPV-Ts can kill the larvae of Thosea sinensis Walker.The LC50of OxocNPV-Ts to such larvae is 4×10^(6) PIB/mL.It is an ideal agent for biological control.We sequenced and characterized the OxocNPV-Ts genome.It was 114,781 bp in length and contained 124 open reading frames.It was most closely related to Oxyplax ochracea nucleopolyhedrovirus(OxocNPV),with a sequence identity of 99%.Phylogenetic analyses showed that OxocNPV-Ts belonged to alpha baculovirus of Baculoviridae according to the species classification standard of the International Committee on Taxonomy of Viruses.OxocNPV-Ts is a distinct strain of OxocNPV.This newly reported genome provides a basis for studies of the molecular mechanisms underlying the virulence of this species.

关 键 词：扁刺蛾核型多角体病毒 基因组 测序分析 

分 类 号：S852.65[农业科学—基础兽医学]