响应面优化黄花菜多酚提取工艺及其抗氧化活性研究  

Optimization of polyphenol extraction from Hemerocallis fulva with response surface methodology and its antioxidant activity

作　　者：张腊腊[1] 胡浩斌[1] 韩明虎[1] 王玉峰[1] 武芸[1] ZHANG Lala;HU Haobin;HAN Minghu;WANG Yufeng;WU Yun(College of Chemistry and Chemical Engineering,Longdong University,Qingyang 745000)

机构地区：[1]陇东学院化学化工学院,庆阳745000

出　　处：《中国食品添加剂》2023年第2期102-108,共7页China Food Additives

基　　金：甘肃省科技厅青年基金项目(21JR7RM193);甘肃省高等学校创新能力提升项目(2019A-120);庆阳市自然基金项目(QY2021A-F012)。

摘　　要：以黄花菜为研究对象,采用超声辅助提取黄花菜中多酚,并利用响应面法优化其多酚提取工艺,考察超声时间、料液比以及乙醇浓度和超声温度对其的影响;通过测定黄花菜中多酚对羟基自由基及DPPH自由基的清除率考察其抗氧化活性。结果表明:黄花菜总多酚在超声功率360 W、液料比40∶1mL/g、超声温度40℃以及超声时间36 min和乙醇浓度60%时达到最大值3.97%。响应面模型预测值为3.98%,相对误差为0.25%,实验值与预测值接近,说明此方法用于黄花菜多酚提取是可行的。实验结果表明,黄花菜多酚对羟基自由基和DPPH自由基的半抑制浓度IC50分别为0.1430 mg/mL和0.1280 mg/mL,其对羟基自由基的清除能力优于Vc,对DPPH自由基的清除能力弱于Vc。Hemerocallis fulva was used as raw material,ultrasound assisted extraction was used to extract polyphenols from Hemerocallis fulva,response surface method was used to optimize the extraction technology. The effects of various parameters(ultrasound time,solid–liquid ratio,ethanol concentration,temperature of extraction)on the extraction process was evaluated. The antioxidant activity of the polyphenol from Hemerocallis fulva was analyzed by hydroxyl radical and DPPH methods. The optimum extraction conditions were as follows :ultrasound power 360W,liquid-solid ratio 40∶1(mL/g),extraction temperature 40℃ and time 36min,ethanol concentration 60%. Under these conditions,the extraction rate of polyphenols was 3.97%,The predictive value of response surface model was 3.98%,and the relative error was 0.25%,the experiment value was close to the predicted value,indicating that the method was feasible for extracting polyphenol from Hemerocallis fulva. The results of antioxidant test showed that the IC50 of hydroxyl radical and DPPH radical were 0.1430 mg/mL and 0.1280 mg/mL respectively. The scavenging ability of polyphenol from Hemerocallis fulva on hydroxyl radical was better than that of Vc,and the scavenging ability on DPPH radical was weaker than that of Vc.

关 键 词：黄花菜 多酚 响应面 超声辅助 提取工艺 

分 类 号：TS202.3[轻工技术与工程—食品科学]